Myo/Nog cells

targets for preventing the accumulation of skeletal muscle-like cells in the human lens

Jacquelyn Gerhart, Marvin Greenbaum, Victoria Scheinfeld, Paul G FitzGerald, Mitchell Crawford, Arturo Bravo-Nuevo, Meghan Pitts, Mindy George-Weinstein

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Posterior capsule opacification (PCO) is a vision impairing condition that arises in some patients following cataract surgery. The fibrotic form of PCO is caused by myofibroblasts that may emerge in the lens years after surgery. In the chick embryo lens, myofibroblasts are derived from Myo/Nog cells that are identified by their expression of the skeletal muscle specific transcription factor MyoD, the bone morphogenetic protein inhibitor Noggin, and the epitope recognized by the G8 monoclonal antibody. The goal of this study was to test the hypothesis that depletion of Myo/Nog cells will prevent the accumulation of myofibroblasts in human lens tissue. Myo/Nog cells were present in anterior, equatorial and bow regions of the human lens, cornea and ciliary processes. In anterior lens tissue removed by capsulorhexis, Myo/Nog cells had synthesized myofibroblast and skeletal muscle proteins, including vimentin, MyoD and sarcomeric myosin. Alpha smooth muscle actin (α-SMA) was detected in a subpopulation of Myo/Nog cells. Areas of the capsule denuded of epithelial cells were surrounded by Myo/Nog cells. Some of these cell free areas contained a wrinkle in the capsule. Depletion of Myo/Nog cells eliminated cells expressing skeletal muscle proteins in 5-day cultures but did not affect cells immunoreactive for beaded filament proteins that accumulate in differentiating lens epithelial cells. Transforming growth factor-betas 1 and 2 that mediate an epithelial-mesenchymal transition, did not induce the expression of skeletal muscle proteins in lens cells following Myo/Nog cell depletion. This study demonstrates that Myo/Nog cells in anterior lens tissue removed from cataract patients have undergone a partial differentiation to skeletal muscle. Myo/Nog cells appear to be the source of skeletal muscle-like cells in explants of human lens tissue. Targeting Myo/Nog cells with the G8 antibody during cataract surgery may reduce the incidence of PCO.

Original languageEnglish (US)
Pages (from-to)e95262
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Lens
Muscle Cells
Lenses
Muscle
skeletal muscle
Skeletal Muscle
Cells
Capsules
Muscle Proteins
cells
Myofibroblasts
Tissue
Surgery
Capsule Opacification
cataract
muscle protein
Cataract
surgery
Bone Morphogenetic Proteins
Vimentin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gerhart, J., Greenbaum, M., Scheinfeld, V., FitzGerald, P. G., Crawford, M., Bravo-Nuevo, A., ... George-Weinstein, M. (2014). Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens. PLoS One, 9(4), e95262. https://doi.org/10.1371/journal.pone.0095262

Myo/Nog cells : targets for preventing the accumulation of skeletal muscle-like cells in the human lens. / Gerhart, Jacquelyn; Greenbaum, Marvin; Scheinfeld, Victoria; FitzGerald, Paul G; Crawford, Mitchell; Bravo-Nuevo, Arturo; Pitts, Meghan; George-Weinstein, Mindy.

In: PLoS One, Vol. 9, No. 4, 2014, p. e95262.

Research output: Contribution to journalArticle

Gerhart, J, Greenbaum, M, Scheinfeld, V, FitzGerald, PG, Crawford, M, Bravo-Nuevo, A, Pitts, M & George-Weinstein, M 2014, 'Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens', PLoS One, vol. 9, no. 4, pp. e95262. https://doi.org/10.1371/journal.pone.0095262
Gerhart, Jacquelyn ; Greenbaum, Marvin ; Scheinfeld, Victoria ; FitzGerald, Paul G ; Crawford, Mitchell ; Bravo-Nuevo, Arturo ; Pitts, Meghan ; George-Weinstein, Mindy. / Myo/Nog cells : targets for preventing the accumulation of skeletal muscle-like cells in the human lens. In: PLoS One. 2014 ; Vol. 9, No. 4. pp. e95262.
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