Myocarditis associated with reovirus in Turkey poults

H L Shivaprasad, M. Franca, P. R. Woolcock, R. Nordhausen, J. M. Day, M. Pantin-Jackwood

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Myocarditis associated with reovirus was diagnosed in 17-day-old, male turkey poults, based on virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), demonstration of reovirus antigen in the cytoplasm of mononuclear inflammatory cells and myocytes in the heart by immunohistochemistry (IHC), and reovirus particles in the endoplasmic reticulum of myocytes by transmission electron microscopy (TEM). Clinical signs in the poults included anorexia, growth depression, and increased mortality. Gross lesions in the six poults examined were increased pericardial fluid, mild-to-moderate dilation of right ventricles, pale-yellow myocardium, and ascites. Other lesions in a few birds included mild pulmonary edema, congestion, and pale serosa of the small intestine that had watery contents in their lumens. Microscopically, in the heart, there was mild-to-severe necrosis of myocytes and infiltration of primarily lymphocytes mixed with a few heterophils, macrophages, and occasionally, plasma cells and multinucleated giant cells. There was mild-to-moderate lymphoid depletion in the bursa of Fabricius. Reovirus was isolated from the heart of the turkey poults in chicken-embryo liver cells and was confirmed by RT-PCR, IHC, and TEM. A retrospective search of the laboratory database for cases of myocarditis associated with reovirus in turkeys revealed that this condition has occurred sporadically in California turkey flocks since 1991. This is the first documentation of myocarditis in turkey poults associated with reovirus.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalAvian Diseases
Volume53
Issue number4
DOIs
StatePublished - 2009

Fingerprint

myocarditis
Reoviridae
poults
Myocarditis
Turkey
Turkey (country)
Muscle Cells
myocytes
Reverse Transcriptase Polymerase Chain Reaction
Transmission Electron Microscopy
heart
lesions (animal)
Immunohistochemistry
immunohistochemistry
transmission electron microscopy
Bursa of Fabricius
Serous Membrane
reverse transcriptase polymerase chain reaction
Anorexia
serosa

Keywords

  • Immunohistochemistry
  • Myocarditis
  • Polymerase chain reaction
  • Reovirus
  • Turkeys

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Shivaprasad, H. L., Franca, M., Woolcock, P. R., Nordhausen, R., Day, J. M., & Pantin-Jackwood, M. (2009). Myocarditis associated with reovirus in Turkey poults. Avian Diseases, 53(4), 523-532. https://doi.org/10.1637/8916-050309-Reg.1

Myocarditis associated with reovirus in Turkey poults. / Shivaprasad, H L; Franca, M.; Woolcock, P. R.; Nordhausen, R.; Day, J. M.; Pantin-Jackwood, M.

In: Avian Diseases, Vol. 53, No. 4, 2009, p. 523-532.

Research output: Contribution to journalArticle

Shivaprasad, HL, Franca, M, Woolcock, PR, Nordhausen, R, Day, JM & Pantin-Jackwood, M 2009, 'Myocarditis associated with reovirus in Turkey poults', Avian Diseases, vol. 53, no. 4, pp. 523-532. https://doi.org/10.1637/8916-050309-Reg.1
Shivaprasad HL, Franca M, Woolcock PR, Nordhausen R, Day JM, Pantin-Jackwood M. Myocarditis associated with reovirus in Turkey poults. Avian Diseases. 2009;53(4):523-532. https://doi.org/10.1637/8916-050309-Reg.1
Shivaprasad, H L ; Franca, M. ; Woolcock, P. R. ; Nordhausen, R. ; Day, J. M. ; Pantin-Jackwood, M. / Myocarditis associated with reovirus in Turkey poults. In: Avian Diseases. 2009 ; Vol. 53, No. 4. pp. 523-532.
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