Myc influences global chromatin structure

Paul S Knoepfler, Xiao Yong Zhang, Pei Feng Cheng, Philip R. Gafken, Steven B. McMahon, Robert N. Eisenman

Research output: Contribution to journalArticlepeer-review

308 Scopus citations


The family of myc proto-oncogenes encodes transcription factors (c-, N-, and L-Myc) that regulate cell growth and proliferation and are involved in the etiology of diverse cancers. Myc proteins are thought to function by binding and regulating specific target genes. Here we report that Myc proteins are required for the widespread maintenance of active chromatin. Disruption of N-myc in neuronal progenitors and other cell types leads to nuclear condensation accompanied by large-scale changes in histone modifications associated with chromatin inactivation, including hypoacetylation and altered methylation. These effects are largely reversed by exogenous Myc as well as by differentiation and are mimicked by the Myc antagonist Mad1. The first chromatin changes are evident within 6 h of Myc loss and lead to changes in chromatin structure. Myc widely influences chromatin in part through upregulation of the histone acetyltransferase GCN5. This study provides the first evidence for regulation of global chromatin structure by an oncoprotein and may explain the broad effects of Myc on cell behavior and tumorigenesis.

Original languageEnglish (US)
Pages (from-to)2723-2734
Number of pages12
JournalEMBO Journal
Issue number12
StatePublished - Jun 21 2006
Externally publishedYes


  • Chromatin
  • Epigenetics
  • Histone modification
  • Myc
  • Stem and progenitor cells

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


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