Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine zipper domain

Agnieszka I. Laskowski, Paul S Knoepfler

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In order to elucidate the function of Myc in the maintenance of pluripotency and self-renewal in mouse embryonic stem cells (mESCs), we screened for novel ESC-specific interactors of Myc by mass spectrometry. Undifferentiated embryonic cell transcription factor 1 (Utf1) was identified in the screen as a putative Myc binding protein in mESCs. We found that Myc and Utf1 directly interact. Utf1 is a chromatin-associated factor required for maintaining pluripotency and self-renewal in mESCs. It can also replace c-myc during induced pluripotent stem cell (iPSC) generation with relatively high efficiency, and shares target genes with Myc in mESCs highlighting a potentially redundant functional role between Myc and Utf1. A large region of Utf1 was found to be necessary for direct interaction with N-Myc, while the basic helix-loop-helix leucine zipper domain of N-Myc is necessary for direct interaction with Utf1.

Original languageEnglish (US)
Pages (from-to)551-556
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume435
Issue number4
DOIs
StatePublished - Jun 14 2013

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Keywords

  • MESCs
  • Myc
  • Transcription
  • Utf1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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