Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth

Gina M. DeStefano, Mazen Kurban, Kwame Anyane-Yeboa, Claudia Dall'Armi, Gilbert Di Paolo, Heather Feenstra, Nanette Silverberg, Luis Rohena, Larissa D. López-Cepeda, Vaidehi Jobanputra, Katherine A. Fantauzzo, Maija Ht Kiuru, Marija Tadin-Strapps, Antonio Sobrino, Anna Vitebsky, Dorothy Warburton, Brynn Levy, Julio C. Salas-Alanis, Angela M. Christiano

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Inherited hypertrichoses are rare syndromes characterized by excessive hair growth that does not result from androgen stimulation, and are often associated with additional congenital abnormalities. In this study, we investigated the genetic defect in a case of autosomal recessive congenital generalized hypertrichosis terminalis (CGHT) (OMIM135400) using whole-exome sequencing. We identified a single base pair substitution in the 5′ donor splice site of intron 32 in the ABC lipid transporter gene ABCA5 that leads to aberrant splicing of the transcript and a decrease in protein levels throughout patient hair follicles. The homozygous recessive disruption of ABCA5 leads to reduced lysosome function, which results in an accumulation of autophagosomes, autophagosomal cargos as well as increased endolysosomal cholesterol in CGHT keratinocytes. In an unrelated sporadic case of CGHT, we identified a 1.3 Mb cryptic deletion of chr17q24.2-q24.3 encompassing ABCA5 and found that ABCA5 levels are dramatically reduced throughout patient hair follicles. Collectively, our findings support ABCA5 as a gene underlying the CGHT phenotype and suggest a novel, previously unrecognized role for this gene in regulating hair growth.

Original languageEnglish (US)
Article numbere1004333
JournalPLoS Genetics
Volume10
Issue number5
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

hair
Hair
hairs
transporters
mutation
hair follicles
Cholesterol
cholesterol
Mutation
Hair Follicle
gene
congenital abnormalities
Genes
genes
keratinocytes
lysosomes
genetic disorders
androgens
Exome
RNA Splice Sites

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)
  • Medicine(all)

Cite this

DeStefano, G. M., Kurban, M., Anyane-Yeboa, K., Dall'Armi, C., Di Paolo, G., Feenstra, H., ... Christiano, A. M. (2014). Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth. PLoS Genetics, 10(5), [e1004333]. https://doi.org/10.1371/journal.pgen.1004333

Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth. / DeStefano, Gina M.; Kurban, Mazen; Anyane-Yeboa, Kwame; Dall'Armi, Claudia; Di Paolo, Gilbert; Feenstra, Heather; Silverberg, Nanette; Rohena, Luis; López-Cepeda, Larissa D.; Jobanputra, Vaidehi; Fantauzzo, Katherine A.; Kiuru, Maija Ht; Tadin-Strapps, Marija; Sobrino, Antonio; Vitebsky, Anna; Warburton, Dorothy; Levy, Brynn; Salas-Alanis, Julio C.; Christiano, Angela M.

In: PLoS Genetics, Vol. 10, No. 5, e1004333, 2014.

Research output: Contribution to journalArticle

DeStefano, GM, Kurban, M, Anyane-Yeboa, K, Dall'Armi, C, Di Paolo, G, Feenstra, H, Silverberg, N, Rohena, L, López-Cepeda, LD, Jobanputra, V, Fantauzzo, KA, Kiuru, MH, Tadin-Strapps, M, Sobrino, A, Vitebsky, A, Warburton, D, Levy, B, Salas-Alanis, JC & Christiano, AM 2014, 'Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth', PLoS Genetics, vol. 10, no. 5, e1004333. https://doi.org/10.1371/journal.pgen.1004333
DeStefano GM, Kurban M, Anyane-Yeboa K, Dall'Armi C, Di Paolo G, Feenstra H et al. Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth. PLoS Genetics. 2014;10(5). e1004333. https://doi.org/10.1371/journal.pgen.1004333
DeStefano, Gina M. ; Kurban, Mazen ; Anyane-Yeboa, Kwame ; Dall'Armi, Claudia ; Di Paolo, Gilbert ; Feenstra, Heather ; Silverberg, Nanette ; Rohena, Luis ; López-Cepeda, Larissa D. ; Jobanputra, Vaidehi ; Fantauzzo, Katherine A. ; Kiuru, Maija Ht ; Tadin-Strapps, Marija ; Sobrino, Antonio ; Vitebsky, Anna ; Warburton, Dorothy ; Levy, Brynn ; Salas-Alanis, Julio C. ; Christiano, Angela M. / Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth. In: PLoS Genetics. 2014 ; Vol. 10, No. 5.
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