TY - JOUR
T1 - Mutational analysis of the human immunodeficiency virus
T2 - The orf-B region down-regulates virus replication
AU - Luciw, Paul A
AU - Cheng-Mayer, C.
AU - Levy, J. A.
PY - 1987
Y1 - 1987
N2 - Mutations were made by recombinant DNA techniques in an infectious molecular clone of the human immunodeficiency virus San Francisco isolate 2 (HIV(SF2)) [formerly the prototype isolates of the acquired immunodeficiency syndrome-asociated retrovirus (ARV-2)]. The effect of these changes on the replicative and cytopathologic properties of the virus was studied by transfecting modified virus clones into cultured human cells. Mutations in the gag, pol, env, and tat regions precluded virus replication and cytopathology in lymphoid cells. A mutation in orf-A dramatically reduced but did not abolish virus replication. Mutant viruses with deletions in the orf-B region were highly cytopathic and replicated to ~5-fold higher levels than wild-type virus. They also produced ~5-fold more viral DNA in infected lymphoid cells than did wild-type virus. Thus, the orf-B region may function to down-regulate virus replication. This mutational analysis of the HIV(SF2) genome is a means of assessing genes regulating viral replication and cytopathology.
AB - Mutations were made by recombinant DNA techniques in an infectious molecular clone of the human immunodeficiency virus San Francisco isolate 2 (HIV(SF2)) [formerly the prototype isolates of the acquired immunodeficiency syndrome-asociated retrovirus (ARV-2)]. The effect of these changes on the replicative and cytopathologic properties of the virus was studied by transfecting modified virus clones into cultured human cells. Mutations in the gag, pol, env, and tat regions precluded virus replication and cytopathology in lymphoid cells. A mutation in orf-A dramatically reduced but did not abolish virus replication. Mutant viruses with deletions in the orf-B region were highly cytopathic and replicated to ~5-fold higher levels than wild-type virus. They also produced ~5-fold more viral DNA in infected lymphoid cells than did wild-type virus. Thus, the orf-B region may function to down-regulate virus replication. This mutational analysis of the HIV(SF2) genome is a means of assessing genes regulating viral replication and cytopathology.
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M3 - Article
C2 - 2434956
AN - SCOPUS:0009524520
VL - 84
SP - 1434
EP - 1438
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 5
ER -