Mutants of rous sarcoma virus with extensive deletions of the viral genome

G. S. Martin, K. Radke, S. Hughes, N. Quintrell, J. M. Bishop, H. E. Varmus

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Deletion mutants of Rous sarcoma virus (RSV) have been isolated from a stock of Prague RSV which had been irradiated with ultraviolet light. Quail fibroblasts were infected with irradiated virus and transformed clones isolated by agar suspension culture. Three clones were obtained which did not release any virus particles. Analysis of DNA from these non-producer clones with restriction endonucleases and the Southern DNA transfer technique indicated that the clones carry defective proviruses with deletions of approximately 4 × 106 daltons of proviral DNA. The defective proviruses, which retain the viral transformation (src) gene, contain only 1.7-2.0 × 106 daltons of DNA. Multiple species of viral RNA containing the sequences of the src gene were detected in these clones; some of these RNAs may contain both viral and cellular sequences. The protein product of the src gene, p60src (Brugge and Erikson, 1977), was also synthesized in the nonproducer clones. However these clones did not contain the products of the group-specific antigen (gag), DNA polymerase (pol), or envelope glycoprotein (env) genes, nor did they contain the 35 and 28 S RNA species which are believed to represent the messengers for these viral gene-products. The properties of these mutants indicate that expression of the src gene is sufficient to induce transformation. These clones may represent useful tools for the study of the expression of this region of the genome.

Original languageEnglish (US)
Pages (from-to)530-546
Number of pages17
JournalVirology
Volume96
Issue number2
DOIs
StatePublished - Jul 30 1979

Fingerprint

Rous sarcoma virus
Viral Genome
Clone Cells
src Genes
Proviruses
DNA
RNA
env Genes
Quail
DNA Restriction Enzymes
Viral RNA
Viral Proteins
DNA-Directed DNA Polymerase
Ultraviolet Rays
Virion
Agar
Suspensions
Glycoproteins
Fibroblasts
Genome

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Martin, G. S., Radke, K., Hughes, S., Quintrell, N., Bishop, J. M., & Varmus, H. E. (1979). Mutants of rous sarcoma virus with extensive deletions of the viral genome. Virology, 96(2), 530-546. https://doi.org/10.1016/0042-6822(79)90109-0

Mutants of rous sarcoma virus with extensive deletions of the viral genome. / Martin, G. S.; Radke, K.; Hughes, S.; Quintrell, N.; Bishop, J. M.; Varmus, H. E.

In: Virology, Vol. 96, No. 2, 30.07.1979, p. 530-546.

Research output: Contribution to journalArticle

Martin, GS, Radke, K, Hughes, S, Quintrell, N, Bishop, JM & Varmus, HE 1979, 'Mutants of rous sarcoma virus with extensive deletions of the viral genome', Virology, vol. 96, no. 2, pp. 530-546. https://doi.org/10.1016/0042-6822(79)90109-0
Martin GS, Radke K, Hughes S, Quintrell N, Bishop JM, Varmus HE. Mutants of rous sarcoma virus with extensive deletions of the viral genome. Virology. 1979 Jul 30;96(2):530-546. https://doi.org/10.1016/0042-6822(79)90109-0
Martin, G. S. ; Radke, K. ; Hughes, S. ; Quintrell, N. ; Bishop, J. M. ; Varmus, H. E. / Mutants of rous sarcoma virus with extensive deletions of the viral genome. In: Virology. 1979 ; Vol. 96, No. 2. pp. 530-546.
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