Mutants of feline immunodeficiency virus resistant to 3′ - azido-3′ -deoxythymidine

Kathryn Martin Remington, Bruce Chesebro, Kathy Wehrly, Niels C. Pedersen, Thomas W North

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66 Scopus citations

Abstract

We selected 3′-azido-3′-deoxythymidine (AZT)-resistant mutants of feline immunodeficiency virus (FIV) in a cat cell culture system. The characterization of one of these mutants was facilitated by the development of a focal immunoassay which could accurately measure FIV infectivity. This assay was used to quantitate the susceptibility of FIV to various inhibitors. The AZT-resistant mutant was found to be cross-resistant to 3′-azido-2′,3′-dideoxyuridine and 3′-azido-2′,3′-dideoxyguanosine but remained sensitive to several other inhibitors (2′,3′-dideoxyinosine, 2′,3′-dideoxy-2′,3′-didehydrothymidine, and phosphonoformate). These patterns of cross-resistance and sensitivity were similar to those of the AZT-resistant human immunodeficiency virus (HIV) that has recently been isolated from patients with AIDS (B. A. Larder and S. D. Kemp, Science 246:1155-1158, 1989). Like the AZT-resistant HIV, purified reverse transcriptase from mutant FIV failed to show resistance to the 5′-triphosphate of AZT. This mutant can be used in the FIV model system to study the mechanisms of drug resistance and to determine the pathogenicity of AZT-resistant mutants.

Original languageEnglish (US)
Pages (from-to)308-312
Number of pages5
JournalJournal of Virology
Volume65
Issue number1
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology

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    Remington, K. M., Chesebro, B., Wehrly, K., Pedersen, N. C., & North, T. W. (1991). Mutants of feline immunodeficiency virus resistant to 3′ - azido-3′ -deoxythymidine. Journal of Virology, 65(1), 308-312.