Mutant mice derived by ICSI of evaporatively dried spermatozoa exhibit expected phenotype

Ming Wen Li, Brian Baridon, Amanda Trainor, Esi Djan, Amanda Koehne, Stephen M Griffey, John D. Biggers, Mehmet Toner, Kevin C K Lloyd

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Apolipoprotein E (Apoe)-deficient knockout mice were used to test the hypothesis that mutant mice preserved as evaporatively dried (ED) spermatozoa, stored at -80 °C for 6 months, and then recovered by ICSI will exhibit the same phenotype as before preservation. The birth rate of mice recovered by ICSI of evaporatively dried spermatozoa was lower than that of fresh spermatozoa (17.5 vs 38.0%). Progeny of mice preserved using evaporatively dried spermatozoa were reproductively sound. From these, the second generation of mice produced by natural mating showed lesions typical of APOE deficiency, including severe hypercholesterolemia, hypertriglyceridemia, markedly increased plasma low-density lipoprotein level, and extensive and severe atherosclerotic lesions in the aorta.We conclude that the expected phenotype caused by an induced genetic mutation can be faithfully recapitulated and sustained in subsequent generations of mice preserved and stored as ED spermatozoa and recovered using ICSI. Because it is simpler, faster, and cheaper than conventional (cryopreservation) and nonconventional (freeze-drying) preservation procedures, evaporative drying is a viable, cost-effective, and efficient method for preserving and storing valuable mutant mouse strains.

Original languageEnglish (US)
Pages (from-to)449-453
Number of pages5
JournalReproduction
Volume143
Issue number4
DOIs
StatePublished - Apr 2012

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Fingerprint Dive into the research topics of 'Mutant mice derived by ICSI of evaporatively dried spermatozoa exhibit expected phenotype'. Together they form a unique fingerprint.

  • Cite this

    Li, M. W., Baridon, B., Trainor, A., Djan, E., Koehne, A., Griffey, S. M., Biggers, J. D., Toner, M., & Lloyd, K. C. K. (2012). Mutant mice derived by ICSI of evaporatively dried spermatozoa exhibit expected phenotype. Reproduction, 143(4), 449-453. https://doi.org/10.1530/REP-12-0005