Abstract
The technique of muscle surface pH measurement used clinically was refined for application in animal experimentation. Guidelines for electrode utilization were developed, and sources of error were investigated. In pentobarbital sodium-anesthetized rabbits, during steady-state conditions, the surface pH of the medial gastrocnemius [7.39 ± 0.05 (SD)] was equal to that of the soleus, and both were lower than femoral venous outflow pH (7.42 ± 0.04, P < 0.001), which was lower than arterial pH (7.46 ± 0.05, P << 0.001). With acid-base infusions, the same relationship found during steady-state control conditions (arterial > venous outflow > muscle surface pH) was observed despite large fluctuations in blood pH. Only during forced hypoventilation, when CO2 transiently moved into muscle, did the relationship reverse. In all experiments, muscle surface pH followed venous outflow pH more closely than it followed arterial pH, the soleus more rapidly than the medial gastrocnemius. Results were consistent with the physiological characteristics of the two muscles and demonstrated that muscle surface pH reflects the local pH of the interstitial compartment under the site of electrode placement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-90 |
| Number of pages | 6 |
| Journal | Journal of Applied Physiology Respiratory Environmental and Exercise Physiology |
| Volume | 52 |
| Issue number | 1 |
| State | Published - 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Physiology
Cite this
Muscle surface pH : Measurement technique and responses to acidosis and alkalosis. / Kost, Gerald J.
In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 52, No. 1, 1982, p. 85-90.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Muscle surface pH
T2 - Measurement technique and responses to acidosis and alkalosis
AU - Kost, Gerald J
PY - 1982
Y1 - 1982
N2 - The technique of muscle surface pH measurement used clinically was refined for application in animal experimentation. Guidelines for electrode utilization were developed, and sources of error were investigated. In pentobarbital sodium-anesthetized rabbits, during steady-state conditions, the surface pH of the medial gastrocnemius [7.39 ± 0.05 (SD)] was equal to that of the soleus, and both were lower than femoral venous outflow pH (7.42 ± 0.04, P < 0.001), which was lower than arterial pH (7.46 ± 0.05, P << 0.001). With acid-base infusions, the same relationship found during steady-state control conditions (arterial > venous outflow > muscle surface pH) was observed despite large fluctuations in blood pH. Only during forced hypoventilation, when CO2 transiently moved into muscle, did the relationship reverse. In all experiments, muscle surface pH followed venous outflow pH more closely than it followed arterial pH, the soleus more rapidly than the medial gastrocnemius. Results were consistent with the physiological characteristics of the two muscles and demonstrated that muscle surface pH reflects the local pH of the interstitial compartment under the site of electrode placement.
AB - The technique of muscle surface pH measurement used clinically was refined for application in animal experimentation. Guidelines for electrode utilization were developed, and sources of error were investigated. In pentobarbital sodium-anesthetized rabbits, during steady-state conditions, the surface pH of the medial gastrocnemius [7.39 ± 0.05 (SD)] was equal to that of the soleus, and both were lower than femoral venous outflow pH (7.42 ± 0.04, P < 0.001), which was lower than arterial pH (7.46 ± 0.05, P << 0.001). With acid-base infusions, the same relationship found during steady-state control conditions (arterial > venous outflow > muscle surface pH) was observed despite large fluctuations in blood pH. Only during forced hypoventilation, when CO2 transiently moved into muscle, did the relationship reverse. In all experiments, muscle surface pH followed venous outflow pH more closely than it followed arterial pH, the soleus more rapidly than the medial gastrocnemius. Results were consistent with the physiological characteristics of the two muscles and demonstrated that muscle surface pH reflects the local pH of the interstitial compartment under the site of electrode placement.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019962856&partnerID=8YFLogxK
M3 - Article
C2 - 7061281
AN - SCOPUS:0019962856
VL - 52
SP - 85
EP - 90
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 1
ER -