Murine marrow cellularity and the concept of stem cell competition

Geographic and quantitative determinants in stem cell biology

G. A. Colvin, J. F. Lambert, Mehrdad Abedi, C. C. Hsieh, J. E. Carlson, F. M. Stewart, P. J. Quesenberry

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In unperturbed mice, the marrow cell numbers correlate with the stem cell numbers. High levels of long-term marrow engraftment are obtained with Infusion of high levels of marrow cells in untreated mice. To address the Issue of stem cell competition vs 'opening space', knowledge of total murine marrow cellularity and distribution of stem and progenitor cells are necessary. We determined these parameters in different mouse strains. Total cellularity in BALB/c mice was 530± 20 million cells; stable from 8 weeks to 1 year of age. C57BL/6J mice had 466±48 million marrow cells. Using these data, theoretical models of infused marrow (40 million cells) replacing or adding to host marrow give chimerism values of 7.5 and 7.0%, respectively; the observed 8-week engraftment of 40 million male BALB/c marrow cells into female hosts (72 mice) gave a value of 6.91±0.4%. This indicates that syngeneic engraftment is determined by stem cell competition. Our studies demonstrate that most marrow cells, progenitors and engraftable stem cells are in the spine. There was increased concentration of progenitors in the spine. Total marrow harvest for stem cell purification and other experimental purposes was both mouse and cost efficient with over a four-fold decrease in animal use and a financial saving.

Original languageEnglish (US)
Pages (from-to)575-583
Number of pages9
JournalLeukemia
Volume18
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

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Cell Biology
Stem Cells
Bone Marrow
Spine
Cell Count
Chimerism
Inbred C57BL Mouse
Theoretical Models
Costs and Cost Analysis

Keywords

  • Bone marrow transplantation
  • Cell differentiation
  • Hematopoietic stem cells
  • Total marrow cellularity

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Colvin, G. A., Lambert, J. F., Abedi, M., Hsieh, C. C., Carlson, J. E., Stewart, F. M., & Quesenberry, P. J. (2004). Murine marrow cellularity and the concept of stem cell competition: Geographic and quantitative determinants in stem cell biology. Leukemia, 18(3), 575-583. https://doi.org/10.1038/sj.leu.2403268

Murine marrow cellularity and the concept of stem cell competition : Geographic and quantitative determinants in stem cell biology. / Colvin, G. A.; Lambert, J. F.; Abedi, Mehrdad; Hsieh, C. C.; Carlson, J. E.; Stewart, F. M.; Quesenberry, P. J.

In: Leukemia, Vol. 18, No. 3, 03.2004, p. 575-583.

Research output: Contribution to journalArticle

Colvin, G. A. ; Lambert, J. F. ; Abedi, Mehrdad ; Hsieh, C. C. ; Carlson, J. E. ; Stewart, F. M. ; Quesenberry, P. J. / Murine marrow cellularity and the concept of stem cell competition : Geographic and quantitative determinants in stem cell biology. In: Leukemia. 2004 ; Vol. 18, No. 3. pp. 575-583.
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