Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity

Juan Anguita, Stephen W Barthold, Rafal Persinski, Michael N. Hedrick, Christy A. Huy, Roger J. Davis, Richard A. Flavell, Erol Fikrig

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Borrelia burgdorferi, the Lyme disease agent, causes joint inflammation in an experimental murine model. Inflammation occurs, in part, due to the ability of B. burgdorferi to induce the production of proinflammatory cytokines and a strong CD4+ T helper type 1 response. The mechanisms by which spirochetes induce these responses are not completely known, although transcription factors, such as NF-κB in phagocytic cells, initiate the proinflammatory cytokine burst. We show here that the mitogen-activated protein (MAP) kinase of 38 kDa (p38 MAP kinase) is involved in the proinflammatory cytokine production elicited by B. burgdorferi Ags in phagocytic cells and the development of murine Lyme arthritis. B. burgdorferi Ags activated p38 MAP kinase in vitro, and the use of a specific inhibitor repressed the spirochete-induced production of TNF-α. The infection of mice that are deficient for a specific upstream activator of the kinase, MAP kinase kinase 3, resulted in diminished proinflammatory cytokine production and the development of arthritis, without compromising the ability of CD4+ T cells to respond to borrelial Ags or the production of specific Abs. Overall, these data indicated that the p38 MAP kinase pathway plays an important role in B. burgdorferi-elicited inflammation and point to potential new therapeutic approaches to the treatment of inflammation induced by the spirochete.

Original languageEnglish (US)
Pages (from-to)6352-6357
Number of pages6
JournalJournal of Immunology
Volume168
Issue number12
StatePublished - Jun 15 2002
Externally publishedYes

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Borrelia burgdorferi
Lyme Disease
p38 Mitogen-Activated Protein Kinases
Spirochaetales
Cytokines
Inflammation
Phagocytes
MAP Kinase Kinase Kinase 3
Mitogen-Activated Protein Kinases
Arthritis
Transcription Factors
Theoretical Models
Joints
T-Lymphocytes
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Anguita, J., Barthold, S. W., Persinski, R., Hedrick, M. N., Huy, C. A., Davis, R. J., ... Fikrig, E. (2002). Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity. Journal of Immunology, 168(12), 6352-6357.

Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity. / Anguita, Juan; Barthold, Stephen W; Persinski, Rafal; Hedrick, Michael N.; Huy, Christy A.; Davis, Roger J.; Flavell, Richard A.; Fikrig, Erol.

In: Journal of Immunology, Vol. 168, No. 12, 15.06.2002, p. 6352-6357.

Research output: Contribution to journalArticle

Anguita, J, Barthold, SW, Persinski, R, Hedrick, MN, Huy, CA, Davis, RJ, Flavell, RA & Fikrig, E 2002, 'Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity', Journal of Immunology, vol. 168, no. 12, pp. 6352-6357.
Anguita J, Barthold SW, Persinski R, Hedrick MN, Huy CA, Davis RJ et al. Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity. Journal of Immunology. 2002 Jun 15;168(12):6352-6357.
Anguita, Juan ; Barthold, Stephen W ; Persinski, Rafal ; Hedrick, Michael N. ; Huy, Christy A. ; Davis, Roger J. ; Flavell, Richard A. ; Fikrig, Erol. / Murine lyme arthritis development mediated by p38 mitogen-activated protein kinase activity. In: Journal of Immunology. 2002 ; Vol. 168, No. 12. pp. 6352-6357.
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