Murine common acute lymphoblastic leukemia antigen (CD10 neutral endopeptidase 24.11)

Molecular characterization, chromosomal localization, and modeling of the active site

Chang Yan Chen, Gilles Salles, Michael F Seldin, Alexander E. Kister, Ellis L. Reinherz, Margaret A. Shipp

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To further analyze CD10/NEP function in lymphoid and nonlymphoid cells using well characterized murine systems, we isolated the murine CD10/NEP homologue, determined its chromosomal location, and modeled the enzyme's active site. The murine CD10/NEP cDNA predicts a 750-amino acid (aa) type II integral membrane protein with 90% identity to the human CD10 sequence and 100% conservation of critical aa and functional motifs. The latter include the pentapeptide consensus sequence required for zinc binding and catalytic activity, additional aa associated with substrate binding, and the extracellular cysteines that participate in disulfide bonds required for enzymatic activity. Like its human homologue, murine CD10/NEP has multiple alternative 5′-untranslated region sequences. The gene is localized on the proximal half of murine chromosome 3. In Northern analysis, murine CD10/ NEP transcripts are abundant in bone marrow stromal cells that support pre-B cell differentiation but are undetectable in representative Abelson transformed pre-B cell lines. The murine CD10/NEP active site was modeled by aligning critical conserved CD10/NEP residues with comparable residues in the active site of thermolysin, a bacterial metalloprotease with similar substrate specificity. The model predicts that the two enzymes have similar clefts that comprise the active site and permit zinc-dependent substrate interactions.

Original languageEnglish (US)
Pages (from-to)2817-2825
Number of pages9
JournalJournal of Immunology
Volume148
Issue number9
StatePublished - May 1 1992
Externally publishedYes

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Neprilysin
Catalytic Domain
B-Lymphoid Precursor Cells
Zinc
Thermolysin
Amino Acids
Amino Acid Motifs
Chromosomes, Human, Pair 3
5' Untranslated Regions
Consensus Sequence
Metalloproteases
Enzymes
Substrate Specificity
Mesenchymal Stromal Cells
Disulfides
Cysteine
Cell Differentiation
Membrane Proteins
Complementary DNA
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Murine common acute lymphoblastic leukemia antigen (CD10 neutral endopeptidase 24.11) : Molecular characterization, chromosomal localization, and modeling of the active site. / Chen, Chang Yan; Salles, Gilles; Seldin, Michael F; Kister, Alexander E.; Reinherz, Ellis L.; Shipp, Margaret A.

In: Journal of Immunology, Vol. 148, No. 9, 01.05.1992, p. 2817-2825.

Research output: Contribution to journalArticle

Chen, Chang Yan ; Salles, Gilles ; Seldin, Michael F ; Kister, Alexander E. ; Reinherz, Ellis L. ; Shipp, Margaret A. / Murine common acute lymphoblastic leukemia antigen (CD10 neutral endopeptidase 24.11) : Molecular characterization, chromosomal localization, and modeling of the active site. In: Journal of Immunology. 1992 ; Vol. 148, No. 9. pp. 2817-2825.
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