Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6

Arthur De Lorimier, Wyatt Byrd, Eric R. Hall, William M. Vaughan, Douglas Tang, Zachary J. Roberts, Charles E. McQueen, Frederick J. Cassels

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Enterotoxigenic Escherichia coli (ETEC) is the most common cause of bacterial diarrhea worldwide and is an important cause of infant morbidity and mortality in developing nations. ETEC colonization factors (CF) are virulence determinants that appear to be protective antigens in humans and are the major target of vaccine efforts. One of the most prevalent CF, CS6, is expressed by about 30% of ETEC worldwide. This study was designed to compare the immunogenicity between encapsulated CS6 (CS6-PLG) and unencapsulated CS6. Recombinant CS6 was purified and encapsulated in biodegradable poly(DL-lactide-co-glycolide) (PLG) microspheres using current Good Manufacturing Practices (cGMP). CS6-PLG and CS6 were administered intranasally (IN) to BALB/c mice in three vaccinations 4 weeks apart. Enzyme linked immunosorbent assay (ELISA) was used to measure the anti-CS6 response in serum and mucosal secretions following each of the three inoculations. Mice vaccinated with two or three doses of CS6-PLG demonstrated a significantly greater rise in serum anti-CS6 IgG and mucosal IgA titer values than those immunized with two or three doses of CS6 alone. Three doses of CS6-PLG led to anti-CS6 serum IgG and mucosal IgA titer values 14-fold and 4.4-fold greater, respectively, than three doses of CS6 (P<0.02). IN administered CS6 to mice is safe and highly immunogenic either alone or when encapsulated in microspheres. PLG microsphere encapsulation of CS6 significantly augments the antibody response to that antigen when administered to a mucosal surface.

Original languageEnglish (US)
Pages (from-to)2548-2555
Number of pages8
JournalVaccine
Volume21
Issue number19-20
DOIs
StatePublished - Jun 2 2003
Externally publishedYes

Fingerprint

Enterotoxigenic Escherichia coli
enterotoxigenic Escherichia coli
Microspheres
Antibody Formation
Immunoglobulin A
antibodies
mice
dosage
blood serum
Serum
Antigens
Polyglactin 910
vaccination
Infant Mortality
Virulence Factors
antigens
good manufacturing practices
Developing Countries
biodegradability
Diarrhea

Keywords

  • Colonization factor
  • Enterotoxigenic E. coli
  • Microspheres

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

De Lorimier, A., Byrd, W., Hall, E. R., Vaughan, W. M., Tang, D., Roberts, Z. J., ... Cassels, F. J. (2003). Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6. Vaccine, 21(19-20), 2548-2555. https://doi.org/10.1016/S0264-410X(03)00101-4

Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6. / De Lorimier, Arthur; Byrd, Wyatt; Hall, Eric R.; Vaughan, William M.; Tang, Douglas; Roberts, Zachary J.; McQueen, Charles E.; Cassels, Frederick J.

In: Vaccine, Vol. 21, No. 19-20, 02.06.2003, p. 2548-2555.

Research output: Contribution to journalArticle

De Lorimier, A, Byrd, W, Hall, ER, Vaughan, WM, Tang, D, Roberts, ZJ, McQueen, CE & Cassels, FJ 2003, 'Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6', Vaccine, vol. 21, no. 19-20, pp. 2548-2555. https://doi.org/10.1016/S0264-410X(03)00101-4
De Lorimier, Arthur ; Byrd, Wyatt ; Hall, Eric R. ; Vaughan, William M. ; Tang, Douglas ; Roberts, Zachary J. ; McQueen, Charles E. ; Cassels, Frederick J. / Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6. In: Vaccine. 2003 ; Vol. 21, No. 19-20. pp. 2548-2555.
@article{e9fae14cc2b8422c80ffba19624fe85c,
title = "Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6",
abstract = "Enterotoxigenic Escherichia coli (ETEC) is the most common cause of bacterial diarrhea worldwide and is an important cause of infant morbidity and mortality in developing nations. ETEC colonization factors (CF) are virulence determinants that appear to be protective antigens in humans and are the major target of vaccine efforts. One of the most prevalent CF, CS6, is expressed by about 30{\%} of ETEC worldwide. This study was designed to compare the immunogenicity between encapsulated CS6 (CS6-PLG) and unencapsulated CS6. Recombinant CS6 was purified and encapsulated in biodegradable poly(DL-lactide-co-glycolide) (PLG) microspheres using current Good Manufacturing Practices (cGMP). CS6-PLG and CS6 were administered intranasally (IN) to BALB/c mice in three vaccinations 4 weeks apart. Enzyme linked immunosorbent assay (ELISA) was used to measure the anti-CS6 response in serum and mucosal secretions following each of the three inoculations. Mice vaccinated with two or three doses of CS6-PLG demonstrated a significantly greater rise in serum anti-CS6 IgG and mucosal IgA titer values than those immunized with two or three doses of CS6 alone. Three doses of CS6-PLG led to anti-CS6 serum IgG and mucosal IgA titer values 14-fold and 4.4-fold greater, respectively, than three doses of CS6 (P<0.02). IN administered CS6 to mice is safe and highly immunogenic either alone or when encapsulated in microspheres. PLG microsphere encapsulation of CS6 significantly augments the antibody response to that antigen when administered to a mucosal surface.",
keywords = "Colonization factor, Enterotoxigenic E. coli, Microspheres",
author = "{De Lorimier}, Arthur and Wyatt Byrd and Hall, {Eric R.} and Vaughan, {William M.} and Douglas Tang and Roberts, {Zachary J.} and McQueen, {Charles E.} and Cassels, {Frederick J.}",
year = "2003",
month = "6",
day = "2",
doi = "10.1016/S0264-410X(03)00101-4",
language = "English (US)",
volume = "21",
pages = "2548--2555",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "19-20",

}

TY - JOUR

T1 - Murine antibody response to intranasally administered enterotoxigenic Escherichia coli colonization factor CS6

AU - De Lorimier, Arthur

AU - Byrd, Wyatt

AU - Hall, Eric R.

AU - Vaughan, William M.

AU - Tang, Douglas

AU - Roberts, Zachary J.

AU - McQueen, Charles E.

AU - Cassels, Frederick J.

PY - 2003/6/2

Y1 - 2003/6/2

N2 - Enterotoxigenic Escherichia coli (ETEC) is the most common cause of bacterial diarrhea worldwide and is an important cause of infant morbidity and mortality in developing nations. ETEC colonization factors (CF) are virulence determinants that appear to be protective antigens in humans and are the major target of vaccine efforts. One of the most prevalent CF, CS6, is expressed by about 30% of ETEC worldwide. This study was designed to compare the immunogenicity between encapsulated CS6 (CS6-PLG) and unencapsulated CS6. Recombinant CS6 was purified and encapsulated in biodegradable poly(DL-lactide-co-glycolide) (PLG) microspheres using current Good Manufacturing Practices (cGMP). CS6-PLG and CS6 were administered intranasally (IN) to BALB/c mice in three vaccinations 4 weeks apart. Enzyme linked immunosorbent assay (ELISA) was used to measure the anti-CS6 response in serum and mucosal secretions following each of the three inoculations. Mice vaccinated with two or three doses of CS6-PLG demonstrated a significantly greater rise in serum anti-CS6 IgG and mucosal IgA titer values than those immunized with two or three doses of CS6 alone. Three doses of CS6-PLG led to anti-CS6 serum IgG and mucosal IgA titer values 14-fold and 4.4-fold greater, respectively, than three doses of CS6 (P<0.02). IN administered CS6 to mice is safe and highly immunogenic either alone or when encapsulated in microspheres. PLG microsphere encapsulation of CS6 significantly augments the antibody response to that antigen when administered to a mucosal surface.

AB - Enterotoxigenic Escherichia coli (ETEC) is the most common cause of bacterial diarrhea worldwide and is an important cause of infant morbidity and mortality in developing nations. ETEC colonization factors (CF) are virulence determinants that appear to be protective antigens in humans and are the major target of vaccine efforts. One of the most prevalent CF, CS6, is expressed by about 30% of ETEC worldwide. This study was designed to compare the immunogenicity between encapsulated CS6 (CS6-PLG) and unencapsulated CS6. Recombinant CS6 was purified and encapsulated in biodegradable poly(DL-lactide-co-glycolide) (PLG) microspheres using current Good Manufacturing Practices (cGMP). CS6-PLG and CS6 were administered intranasally (IN) to BALB/c mice in three vaccinations 4 weeks apart. Enzyme linked immunosorbent assay (ELISA) was used to measure the anti-CS6 response in serum and mucosal secretions following each of the three inoculations. Mice vaccinated with two or three doses of CS6-PLG demonstrated a significantly greater rise in serum anti-CS6 IgG and mucosal IgA titer values than those immunized with two or three doses of CS6 alone. Three doses of CS6-PLG led to anti-CS6 serum IgG and mucosal IgA titer values 14-fold and 4.4-fold greater, respectively, than three doses of CS6 (P<0.02). IN administered CS6 to mice is safe and highly immunogenic either alone or when encapsulated in microspheres. PLG microsphere encapsulation of CS6 significantly augments the antibody response to that antigen when administered to a mucosal surface.

KW - Colonization factor

KW - Enterotoxigenic E. coli

KW - Microspheres

UR - http://www.scopus.com/inward/record.url?scp=0038669207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038669207&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(03)00101-4

DO - 10.1016/S0264-410X(03)00101-4

M3 - Article

C2 - 12744890

AN - SCOPUS:0038669207

VL - 21

SP - 2548

EP - 2555

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 19-20

ER -