Abstract
A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.
Original language | English (US) |
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Pages (from-to) | 16950-16962 |
Number of pages | 13 |
Journal | Optics Express |
Volume | 19 |
Issue number | 18 |
DOIs | |
State | Published - Aug 29 2011 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics