Abstract
A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 16950-16962 |
| Number of pages | 13 |
| Journal | Optics Express |
| Volume | 19 |
| Issue number | 18 |
| DOIs | |
| State | Published - Aug 29 2011 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy. / Smith, Zachary J.; Strombom, Sven; Wachsmann-Hogiu, Sebastian.
In: Optics Express, Vol. 19, No. 18, 29.08.2011, p. 16950-16962.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy
AU - Smith, Zachary J.
AU - Strombom, Sven
AU - Wachsmann-Hogiu, Sebastian
PY - 2011/8/29
Y1 - 2011/8/29
N2 - A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.
AB - A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.
UR - http://www.scopus.com/inward/record.url?scp=80052198698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052198698&partnerID=8YFLogxK
U2 - 10.1364/OE.19.016950
DO - 10.1364/OE.19.016950
M3 - Article
C2 - 21935055
AN - SCOPUS:80052198698
VL - 19
SP - 16950
EP - 16962
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 18
ER -