Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy

Zachary J. Smith, Sven Strombom, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.

Original languageEnglish (US)
Pages (from-to)16950-16962
Number of pages13
JournalOptics Express
Volume19
Issue number18
DOIs
StatePublished - Aug 29 2011

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Raman spectroscopy
optical computers
fluorescence
photomultiplier tubes
spectroscopy
spectrographs
Raman spectra
detectors
simulation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy. / Smith, Zachary J.; Strombom, Sven; Wachsmann-Hogiu, Sebastian.

In: Optics Express, Vol. 19, No. 18, 29.08.2011, p. 16950-16962.

Research output: Contribution to journalArticle

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