Abstract
A compact, non-invasive multi-modal system has been developed for in vivo mouse retina imaging. It is configured for simultaneously detecting green and red fluorescent protein signals with scanning laser ophthalmoscopy (SLO) back-scattered light from the SLO illumination beam, and depth information about different retinal layers by means of Optical Coherence Tomography (OCT). Simultaneous assessment of retinal characteristics with different modalities can provide a wealth of information about the structural and functional changes in the retinal neural tissue and chorio-retinal vasculature in vivo. Additionally, simultaneous acquisition of multiple channels facilitates analysis of the data of different modalities by automatic temporal and structural co-registration. As an example of the instrument’s performance we imaged the retina of a mouse with constitutive expression of GFP in microglia cells (Cx3cr1<sup>GFP/+</sup>), and which also expressed the red fluorescent protein mCherry in Müller glial cells by means of adeno-Associated virus delivery (AAV2) of an mCherry cDNA driven by the GFAP (glial fibrillary acid protein) promoter.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Publisher | SPIE |
| Volume | 9307 |
| ISBN (Print) | 9781628413977 |
| DOIs | |
| State | Published - 2015 |
| Event | 25th Conference on Ophthalmic Technologies - San Francisco, United States Duration: Feb 7 2015 → Feb 8 2015 |
Other
| Other | 25th Conference on Ophthalmic Technologies |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/7/15 → 2/8/15 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging. / Zhang, Pengfei; Zam, Azhar; Jian, Yifan; Wang, Xinlei; Burns, Marie E; Sarunic, Marinko V.; Pugh Jr, Edward N; Zawadzki, Robert.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9307 SPIE, 2015. 93070H.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multispectral scanning laser ophthalmoscopy combined with optical coherence tomography for simultaneous in vivo mouse retinal imaging
AU - Zhang, Pengfei
AU - Zam, Azhar
AU - Jian, Yifan
AU - Wang, Xinlei
AU - Burns, Marie E
AU - Sarunic, Marinko V.
AU - Pugh Jr, Edward N
AU - Zawadzki, Robert
PY - 2015
Y1 - 2015
N2 - A compact, non-invasive multi-modal system has been developed for in vivo mouse retina imaging. It is configured for simultaneously detecting green and red fluorescent protein signals with scanning laser ophthalmoscopy (SLO) back-scattered light from the SLO illumination beam, and depth information about different retinal layers by means of Optical Coherence Tomography (OCT). Simultaneous assessment of retinal characteristics with different modalities can provide a wealth of information about the structural and functional changes in the retinal neural tissue and chorio-retinal vasculature in vivo. Additionally, simultaneous acquisition of multiple channels facilitates analysis of the data of different modalities by automatic temporal and structural co-registration. As an example of the instrument’s performance we imaged the retina of a mouse with constitutive expression of GFP in microglia cells (Cx3cr1GFP/+), and which also expressed the red fluorescent protein mCherry in Müller glial cells by means of adeno-Associated virus delivery (AAV2) of an mCherry cDNA driven by the GFAP (glial fibrillary acid protein) promoter.
AB - A compact, non-invasive multi-modal system has been developed for in vivo mouse retina imaging. It is configured for simultaneously detecting green and red fluorescent protein signals with scanning laser ophthalmoscopy (SLO) back-scattered light from the SLO illumination beam, and depth information about different retinal layers by means of Optical Coherence Tomography (OCT). Simultaneous assessment of retinal characteristics with different modalities can provide a wealth of information about the structural and functional changes in the retinal neural tissue and chorio-retinal vasculature in vivo. Additionally, simultaneous acquisition of multiple channels facilitates analysis of the data of different modalities by automatic temporal and structural co-registration. As an example of the instrument’s performance we imaged the retina of a mouse with constitutive expression of GFP in microglia cells (Cx3cr1GFP/+), and which also expressed the red fluorescent protein mCherry in Müller glial cells by means of adeno-Associated virus delivery (AAV2) of an mCherry cDNA driven by the GFAP (glial fibrillary acid protein) promoter.
UR - http://www.scopus.com/inward/record.url?scp=84928556504&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928556504&partnerID=8YFLogxK
U2 - 10.1117/12.2080468
DO - 10.1117/12.2080468
M3 - Conference contribution
AN - SCOPUS:84928556504
SN - 9781628413977
VL - 9307
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
PB - SPIE
ER -