Multispectral imaging in biology and medicine: Slices of life

Richard M Levenson, James R. Mansfield

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Multispectral imaging (MSI) is currently in a period of transition from its role as an exotic technique to its being offered in one form or another by all the major microscopy manufacturers. This is because it provides solutions to some of the major challenges in fluorescence-based imaging, namely ameliorating the consequences of the presence of autofluorescence and the need to easily accommodate relatively high levels of signal multiplexing. MSI, which spectrally characterizes and computationally eliminates autofluorescence, enhances the signal-to-background dramatically, revealing otherwise obscured targets. While this article concentrates on examples derived from liquid-crystal tunable filter-based technology, the intent is to showcase the advantages of multispectral imaging in general. Some technologies used to generate multispectral images are compatible with only particular optical configurations, such as point-scanning laser confocal microscopy. Band-sequential approaches, such as those afforded by liquid-crystal tunable filters (LCTFs), can be conveniently coupled with a variety of imaging modalities, which, in addition to fluorescence microscopy, include brightfield (nonfluorescent) microscopy as well as small-animal, noninvasive in-vivo imaging. Brightfield microscopy is the chosen format for histopathology, which relies on immunohistochemistry to provide molecularly resolved clinical information. However, in contrast to fluorescent labels, multiple chromogens, if they spatially overlap, are much harder to separate and quantitate, unless MSI approaches are used. In-vivo imaging is a rapidly growing field with applications in basic biology, drug discovery, and clinical medicine. The sensitivity of fluorescence-based in-vivo imaging, as with fluorescence microscopy, can be limited by the presence of significant autofluorescence, a limitation which can be overcome through the utilization of MSI.

Original languageEnglish (US)
Pages (from-to)748-758
Number of pages11
JournalCytometry Part A
Volume69
Issue number8
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Fingerprint

Microscopy
Liquid Crystals
Medicine
Fluorescence Microscopy
Technology
Optical Imaging
Clinical Medicine
Drug Discovery
Confocal Microscopy
Fluorescence
Immunohistochemistry

Keywords

  • Autofluorescence
  • Fluorescence
  • Immunohistochemistry
  • In-vivo imaging
  • Multiplexing

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Multispectral imaging in biology and medicine : Slices of life. / Levenson, Richard M; Mansfield, James R.

In: Cytometry Part A, Vol. 69, No. 8, 01.08.2006, p. 748-758.

Research output: Contribution to journalArticle

Levenson, Richard M ; Mansfield, James R. / Multispectral imaging in biology and medicine : Slices of life. In: Cytometry Part A. 2006 ; Vol. 69, No. 8. pp. 748-758.
@article{17e243cf181e436d94fb2501efe1fb75,
title = "Multispectral imaging in biology and medicine: Slices of life",
abstract = "Multispectral imaging (MSI) is currently in a period of transition from its role as an exotic technique to its being offered in one form or another by all the major microscopy manufacturers. This is because it provides solutions to some of the major challenges in fluorescence-based imaging, namely ameliorating the consequences of the presence of autofluorescence and the need to easily accommodate relatively high levels of signal multiplexing. MSI, which spectrally characterizes and computationally eliminates autofluorescence, enhances the signal-to-background dramatically, revealing otherwise obscured targets. While this article concentrates on examples derived from liquid-crystal tunable filter-based technology, the intent is to showcase the advantages of multispectral imaging in general. Some technologies used to generate multispectral images are compatible with only particular optical configurations, such as point-scanning laser confocal microscopy. Band-sequential approaches, such as those afforded by liquid-crystal tunable filters (LCTFs), can be conveniently coupled with a variety of imaging modalities, which, in addition to fluorescence microscopy, include brightfield (nonfluorescent) microscopy as well as small-animal, noninvasive in-vivo imaging. Brightfield microscopy is the chosen format for histopathology, which relies on immunohistochemistry to provide molecularly resolved clinical information. However, in contrast to fluorescent labels, multiple chromogens, if they spatially overlap, are much harder to separate and quantitate, unless MSI approaches are used. In-vivo imaging is a rapidly growing field with applications in basic biology, drug discovery, and clinical medicine. The sensitivity of fluorescence-based in-vivo imaging, as with fluorescence microscopy, can be limited by the presence of significant autofluorescence, a limitation which can be overcome through the utilization of MSI.",
keywords = "Autofluorescence, Fluorescence, Immunohistochemistry, In-vivo imaging, Multiplexing",
author = "Levenson, {Richard M} and Mansfield, {James R.}",
year = "2006",
month = "8",
day = "1",
doi = "10.1002/cyto.a.20319",
language = "English (US)",
volume = "69",
pages = "748--758",
journal = "Cytometry. Part A : the journal of the International Society for Analytical Cytology",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Multispectral imaging in biology and medicine

T2 - Slices of life

AU - Levenson, Richard M

AU - Mansfield, James R.

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Multispectral imaging (MSI) is currently in a period of transition from its role as an exotic technique to its being offered in one form or another by all the major microscopy manufacturers. This is because it provides solutions to some of the major challenges in fluorescence-based imaging, namely ameliorating the consequences of the presence of autofluorescence and the need to easily accommodate relatively high levels of signal multiplexing. MSI, which spectrally characterizes and computationally eliminates autofluorescence, enhances the signal-to-background dramatically, revealing otherwise obscured targets. While this article concentrates on examples derived from liquid-crystal tunable filter-based technology, the intent is to showcase the advantages of multispectral imaging in general. Some technologies used to generate multispectral images are compatible with only particular optical configurations, such as point-scanning laser confocal microscopy. Band-sequential approaches, such as those afforded by liquid-crystal tunable filters (LCTFs), can be conveniently coupled with a variety of imaging modalities, which, in addition to fluorescence microscopy, include brightfield (nonfluorescent) microscopy as well as small-animal, noninvasive in-vivo imaging. Brightfield microscopy is the chosen format for histopathology, which relies on immunohistochemistry to provide molecularly resolved clinical information. However, in contrast to fluorescent labels, multiple chromogens, if they spatially overlap, are much harder to separate and quantitate, unless MSI approaches are used. In-vivo imaging is a rapidly growing field with applications in basic biology, drug discovery, and clinical medicine. The sensitivity of fluorescence-based in-vivo imaging, as with fluorescence microscopy, can be limited by the presence of significant autofluorescence, a limitation which can be overcome through the utilization of MSI.

AB - Multispectral imaging (MSI) is currently in a period of transition from its role as an exotic technique to its being offered in one form or another by all the major microscopy manufacturers. This is because it provides solutions to some of the major challenges in fluorescence-based imaging, namely ameliorating the consequences of the presence of autofluorescence and the need to easily accommodate relatively high levels of signal multiplexing. MSI, which spectrally characterizes and computationally eliminates autofluorescence, enhances the signal-to-background dramatically, revealing otherwise obscured targets. While this article concentrates on examples derived from liquid-crystal tunable filter-based technology, the intent is to showcase the advantages of multispectral imaging in general. Some technologies used to generate multispectral images are compatible with only particular optical configurations, such as point-scanning laser confocal microscopy. Band-sequential approaches, such as those afforded by liquid-crystal tunable filters (LCTFs), can be conveniently coupled with a variety of imaging modalities, which, in addition to fluorescence microscopy, include brightfield (nonfluorescent) microscopy as well as small-animal, noninvasive in-vivo imaging. Brightfield microscopy is the chosen format for histopathology, which relies on immunohistochemistry to provide molecularly resolved clinical information. However, in contrast to fluorescent labels, multiple chromogens, if they spatially overlap, are much harder to separate and quantitate, unless MSI approaches are used. In-vivo imaging is a rapidly growing field with applications in basic biology, drug discovery, and clinical medicine. The sensitivity of fluorescence-based in-vivo imaging, as with fluorescence microscopy, can be limited by the presence of significant autofluorescence, a limitation which can be overcome through the utilization of MSI.

KW - Autofluorescence

KW - Fluorescence

KW - Immunohistochemistry

KW - In-vivo imaging

KW - Multiplexing

UR - http://www.scopus.com/inward/record.url?scp=33749143839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749143839&partnerID=8YFLogxK

U2 - 10.1002/cyto.a.20319

DO - 10.1002/cyto.a.20319

M3 - Article

C2 - 16969820

AN - SCOPUS:33749143839

VL - 69

SP - 748

EP - 758

JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology

JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology

SN - 1552-4922

IS - 8

ER -