Multispectral fluorescence lifetime imaging system for intravascular diagnostics with ultrasound guidance: In vivo validation in swine arteries

Julien Bec, Dinglong M. Ma, Diego R. Yankelevich, Jing Liu, William T. Ferrier, Jeffrey Southard, Laura Marcu

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Fluorescence lifetime technique has demonstrated potential for analysis of atherosclerotic lesions and for complementing existing intravascular imaging modalities such as intravascular ultrasound (IVUS) in identifying lesions at high risk of rupture. This study presents a multimodal catheter system integrating a 40 MHz commercial IVUS and fluorescence lifetime imaging (FLIm) using fast helical motion scanning (400 rpm, 0.75 mm/s), able to acquire in vivo in pulsatile blood flow the autofluorescence emission of arterial vessels with high precision (5.08 ± 0.26 ns mean average lifetime over 13 scans). Co-registered FLIm and IVUS data allowed 3D visualization of both biochemical and morphological vessel properties. Current study supports the development of clinically compatible intravascular diagnostic system integrating FLIm and demonstrates, to our knowledge, the first in vivo intravascular application of a fluorescence lifetime imaging technique.

Original languageEnglish (US)
Pages (from-to)281-285
Number of pages5
JournalJournal of Biophotonics
Volume7
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Atherosclerosis
  • Fluorescence lifetime imaging
  • Intravascular catheter
  • Intravascular ultrasound (IVUS)
  • Time resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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