Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector

Xiangnan Zhou, Julien Bec, Diego Yankelevich, Laura Marcu

Research output: Contribution to journalArticlepeer-review

Abstract

We report the design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device incorporating solid-state detectors and automated gain control. For every excitation pulse (~1 μJ, 600 ps), this device records complete fluorescence decay from multiple spectral channels simultaneously within microseconds, using a dedicated UV enhanced avalanche photodetector and analog to digital convert (2.5 GS/s) in each channel. Fast (<2 ms) channel-wise dynamic range adjustment maximizes the signal-to-noise ratio. Fluorophores with known lifetime ranging from 0.5-6.0 ns were used to demonstrate the device accuracy. Current results show the clear benefits of this device compared to existing devices employing microchannel-plate photomultiplier tubes. This is demonstrated by 5-fold reduction of lifetime measurement variability in identical conditions, independent gain adjustment in each spectral band, and 4-times faster imaging speed. The use of solid-state detectors will also facilitate future improved performance and miniaturization of the instrument.

Original languageEnglish (US)
Pages (from-to)20105-20120
Number of pages16
JournalOptics Express
Volume29
Issue number13
DOIs
StatePublished - Jun 21 2021

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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