Abstract
Bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. However, the relationship between human and cat isolates of B. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. Multilocus sequence typing (MLST), the most discriminatory genotyping method for B. henselae, identified seven genotypes and suggested that human isolates arose from a limited number of cat isolates. Herein, we estimated the discriminatory power of multispacer typing (MST) by studying 126 B. henselae cat isolates from various areas of Europe, Asia, and the United States. We identified the nine most variable intergenic spacers conserved by both B. henselae and Bartonella quintana genomes. By comparing the sequences obtained from these nine spacers for each studied isolate, we identified 39 MST genotypes. The distribution of isolates into MST genotypes matched their phylogenetic organization into four clusters. MST showed that European and Asian isolates were different, in contrast with American isolates, but failed to identify pandemic strains. Our study demonstrated that MST is a powerful method for genotyping B. henselae at the strain level and may serve in studying the population dynamics of this bacterium and understanding the relationships between cat and human isolates. Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/ MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2499-2506 |
| Number of pages | 8 |
| Journal | Journal of Clinical Microbiology |
| Volume | 44 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2006 |
Fingerprint
ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
Multispacer typing to study the genotypic distribution of Bartonella henselae populations. / Li, Wenjun; Chomel, Bruno B; Maruyama, Soichi; Guptil, Lynn; Sander, Anna; Raoult, Didier; Fournier, Pierre Edouard.
In: Journal of Clinical Microbiology, Vol. 44, No. 7, 07.2006, p. 2499-2506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multispacer typing to study the genotypic distribution of Bartonella henselae populations
AU - Li, Wenjun
AU - Chomel, Bruno B
AU - Maruyama, Soichi
AU - Guptil, Lynn
AU - Sander, Anna
AU - Raoult, Didier
AU - Fournier, Pierre Edouard
PY - 2006/7
Y1 - 2006/7
N2 - Bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. However, the relationship between human and cat isolates of B. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. Multilocus sequence typing (MLST), the most discriminatory genotyping method for B. henselae, identified seven genotypes and suggested that human isolates arose from a limited number of cat isolates. Herein, we estimated the discriminatory power of multispacer typing (MST) by studying 126 B. henselae cat isolates from various areas of Europe, Asia, and the United States. We identified the nine most variable intergenic spacers conserved by both B. henselae and Bartonella quintana genomes. By comparing the sequences obtained from these nine spacers for each studied isolate, we identified 39 MST genotypes. The distribution of isolates into MST genotypes matched their phylogenetic organization into four clusters. MST showed that European and Asian isolates were different, in contrast with American isolates, but failed to identify pandemic strains. Our study demonstrated that MST is a powerful method for genotyping B. henselae at the strain level and may serve in studying the population dynamics of this bacterium and understanding the relationships between cat and human isolates. Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/ MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database.
AB - Bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. However, the relationship between human and cat isolates of B. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. Multilocus sequence typing (MLST), the most discriminatory genotyping method for B. henselae, identified seven genotypes and suggested that human isolates arose from a limited number of cat isolates. Herein, we estimated the discriminatory power of multispacer typing (MST) by studying 126 B. henselae cat isolates from various areas of Europe, Asia, and the United States. We identified the nine most variable intergenic spacers conserved by both B. henselae and Bartonella quintana genomes. By comparing the sequences obtained from these nine spacers for each studied isolate, we identified 39 MST genotypes. The distribution of isolates into MST genotypes matched their phylogenetic organization into four clusters. MST showed that European and Asian isolates were different, in contrast with American isolates, but failed to identify pandemic strains. Our study demonstrated that MST is a powerful method for genotyping B. henselae at the strain level and may serve in studying the population dynamics of this bacterium and understanding the relationships between cat and human isolates. Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/ MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database.
UR - http://www.scopus.com/inward/record.url?scp=33746216123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746216123&partnerID=8YFLogxK
U2 - 10.1128/JCM.00498-06
DO - 10.1128/JCM.00498-06
M3 - Article
C2 - 16825371
AN - SCOPUS:33746216123
VL - 44
SP - 2499
EP - 2506
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 7
ER -