Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber

Benjamin E. Sherlock, Cai Li, Xiangnan Zhou, Alba Alfonso-Garcia, Julien Bec, Diego Yankelevich, Laura Marcu

Research output: Contribution to journalArticle

Abstract

Fiber-based imaging of tissue autofluorescence using ultraviolet (UV) excitation is a highly flexible tool used to probe structure and composition. In this Letter, we report, to the best of our knowledge, the first results from a single-fiber imaging system employing a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two distinct spatial resolutions. We characterize the lateral point spread function and fluorescent background of the system and show how enhanced resolution can identify features such as trabeculae in ex vivo murine bone samples.

Original languageEnglish (US)
Pages (from-to)2302-2305
Number of pages4
JournalOptics Letters
Volume44
Issue number9
DOIs
StatePublished - May 1 2019

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life (durability)
fluorescence
fibers
point spread functions
bones
spatial resolution
probes
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Sherlock, B. E., Li, C., Zhou, X., Alfonso-Garcia, A., Bec, J., Yankelevich, D., & Marcu, L. (2019). Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber. Optics Letters, 44(9), 2302-2305. https://doi.org/10.1364/OL.44.002302

Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber. / Sherlock, Benjamin E.; Li, Cai; Zhou, Xiangnan; Alfonso-Garcia, Alba; Bec, Julien; Yankelevich, Diego; Marcu, Laura.

In: Optics Letters, Vol. 44, No. 9, 01.05.2019, p. 2302-2305.

Research output: Contribution to journalArticle

Sherlock, BE, Li, C, Zhou, X, Alfonso-Garcia, A, Bec, J, Yankelevich, D & Marcu, L 2019, 'Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber', Optics Letters, vol. 44, no. 9, pp. 2302-2305. https://doi.org/10.1364/OL.44.002302
Sherlock BE, Li C, Zhou X, Alfonso-Garcia A, Bec J, Yankelevich D et al. Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber. Optics Letters. 2019 May 1;44(9):2302-2305. https://doi.org/10.1364/OL.44.002302
Sherlock, Benjamin E. ; Li, Cai ; Zhou, Xiangnan ; Alfonso-Garcia, Alba ; Bec, Julien ; Yankelevich, Diego ; Marcu, Laura. / Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber. In: Optics Letters. 2019 ; Vol. 44, No. 9. pp. 2302-2305.
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