Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells

Jesus Isaac Luna, Jesus Ciriza, Marcos E. Garcia-Ojeda, Marco Kong, Anthony Herren, Deborah Lieu, Ronald A. Li, Charless C. Fowlkes, Michelle Khine, Kara E. McCloskey

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Nano- and microscale topographical cues play critical roles in the induction and maintenance of various cellular functions, including morphology, adhesion, gene regulation, and communication. Recent studies indicate that structure and function at the heart tissue level is exquisitely sensitive to mechanical cues at the nano-scale as well as at the microscale level. Although fabrication methods exist for generating topographical features for cell culture, current techniques, especially those with nanoscale resolution, are typically complex, prohibitively expensive, and not accessible to most biology laboratories. Here, we present a tunable culture platform comprised of biomimetic wrinkles that simulate the heart's complex anisotropic and multiscale architecture for facile and robust cardiac cell alignment. We demonstrate the cellular and subcellular alignment of both neonatal mouse cardiomyocytes as well as those derived from human embryonic stem cells. By mimicking the fibrillar network of the extracellular matrix, this system enables monitoring of protein localization in real time and therefore the high-resolution study of phenotypic and physiologic responses to in-vivo like topographical cues.

Original languageEnglish (US)
Pages (from-to)579-588
Number of pages10
JournalTissue Engineering - Part C: Methods
Volume17
Issue number5
DOIs
StatePublished - May 1 2011

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Biomimetics
Embryonic Stem Cells
Stem cells
Topography
Cues
Cell culture
Gene expression
Adhesion
Tissue
Proteins
Cardiac Myocytes
Fabrication
Extracellular Matrix
Monitoring
Communication
Cell Culture Techniques
Maintenance
Genes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)
  • Medicine(all)

Cite this

Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells. / Luna, Jesus Isaac; Ciriza, Jesus; Garcia-Ojeda, Marcos E.; Kong, Marco; Herren, Anthony; Lieu, Deborah; Li, Ronald A.; Fowlkes, Charless C.; Khine, Michelle; McCloskey, Kara E.

In: Tissue Engineering - Part C: Methods, Vol. 17, No. 5, 01.05.2011, p. 579-588.

Research output: Contribution to journalArticle

Luna, JI, Ciriza, J, Garcia-Ojeda, ME, Kong, M, Herren, A, Lieu, D, Li, RA, Fowlkes, CC, Khine, M & McCloskey, KE 2011, 'Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells', Tissue Engineering - Part C: Methods, vol. 17, no. 5, pp. 579-588. https://doi.org/10.1089/ten.tec.2010.0410
Luna, Jesus Isaac ; Ciriza, Jesus ; Garcia-Ojeda, Marcos E. ; Kong, Marco ; Herren, Anthony ; Lieu, Deborah ; Li, Ronald A. ; Fowlkes, Charless C. ; Khine, Michelle ; McCloskey, Kara E. / Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells. In: Tissue Engineering - Part C: Methods. 2011 ; Vol. 17, No. 5. pp. 579-588.
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