Multiplexed immunoassays for biomonitoring of exposure to agrochemicals using quantum dots as fluorescent reporters

Mikaela Nichkova, Dosi Dosev, Alexander E. Davies, Shirley J. Gee, Ian M. Kennedy, Bruce D. Hammock

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using quantum dots (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6448
DOIs
StatePublished - 2007
EventColloidal Quantum Dots for Biomedical Applications II - San Jose, CA, United States
Duration: Jan 20 2007Jan 23 2007

Other

OtherColloidal Quantum Dots for Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/20/071/23/07

Keywords

  • Atrazine
  • Immunoassay
  • Microarrays
  • Microconatct printing
  • Pyrethroid
  • Quantum dot

ASJC Scopus subject areas

  • Engineering(all)

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    Nichkova, M., Dosev, D., Davies, A. E., Gee, S. J., Kennedy, I. M., & Hammock, B. D. (2007). Multiplexed immunoassays for biomonitoring of exposure to agrochemicals using quantum dots as fluorescent reporters. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6448). [644817] https://doi.org/10.1117/12.699231