Multiplex qRT-PCR for the detection of western equine encephalomyelitis, St. Louis encephalitis, and west nile viral RNA in mosquito pools (diptera: Culicidae)

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15 Citations (Scopus)

Abstract

Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.

Original languageEnglish (US)
Pages (from-to)491-499
Number of pages9
JournalJournal of Medical Entomology
Volume52
Issue number3
DOIs
StatePublished - Jan 1 2015

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Western Equine Encephalomyelitis
St. Louis Encephalitis
Western equine encephalomyelitis
Multiplex Polymerase Chain Reaction
Viral RNA
encephalitis
Culicidae
Diptera
Reverse Transcription
Arbovirus Infections
RNA
West Nile virus
Polymerase Chain Reaction
assays
Disease Outbreaks
Public Health
reverse transcriptase polymerase chain reaction
Viruses
Antigens
Sensitivity and Specificity

Keywords

  • Multiplex
  • St. Louis encephalitis virus
  • Surveillance
  • West Nile virus
  • Western equine encephalitis virus

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)
  • Infectious Diseases
  • Parasitology
  • Medicine(all)

Cite this

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title = "Multiplex qRT-PCR for the detection of western equine encephalomyelitis, St. Louis encephalitis, and west nile viral RNA in mosquito pools (diptera: Culicidae)",
abstract = "Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.",
keywords = "Multiplex, St. Louis encephalitis virus, Surveillance, West Nile virus, Western equine encephalitis virus",
author = "Aaron Brault and Ying Fang and William Reisen",
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T1 - Multiplex qRT-PCR for the detection of western equine encephalomyelitis, St. Louis encephalitis, and west nile viral RNA in mosquito pools (diptera

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AU - Brault, Aaron

AU - Fang, Ying

AU - Reisen, William

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.

AB - Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.

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KW - Surveillance

KW - West Nile virus

KW - Western equine encephalitis virus

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