Multiple, independent T cell lymphomas arising in an experimentally FIV-infected cat during the terminal stage of infection

Brian G Murphy, Christina Eckstrand, Diego Castillo, Andre Poon, Molly Liepnieks, Kristy Harmon, Peter F Moore

Research output: Contribution to journalArticle

Abstract

Our laboratory has serially reported on the virologic and immunopathologic features of a cohort of experimental feline immunodeficiency virus (FIV)-infected cats for more than eight years. At 8.09 years post infection (PI), one of these animals entered the terminal stage of infection, characterized by undulating hyperthermia, progressive anorexia, weight loss, and pancytopenia; the animal was not responsive to therapeutic interventions, necessitating euthanasia six weeks later (8.20 years PI). Subsequent analyses indicated that neoplastic lymphocytes infiltrated multiple cervical lymph nodes and a band-like region of the mucosal lamina propria within a segment of the intestine. Immunohistochemistry and T cell clonality testing determined that the nodal and intestinal lesions were independently arising from CD3 T cell lymphomas. In-situ RNA hybridization studies indicated that diffuse neoplastic lymphocytes from the cervical lymph node contained abundant viral nucleic acid, while viral nucleic acid was not detectable in lymphocytes from the intestinal lymphoma lesion. The proviral long terminal repeat (LTR) was amplified and sequenced from multiple anatomic sites, and a common clone containing a single nucleotide polymorphism was determined to be defective in response to phorbol myristate acetate (PMA)-mediated promoter activation in a reporter gene assay. This assay revealed a previously unidentified PMA response element within the FIV U3 region 3’ to the TATA box. The possible implications of these results on FIV-lymphoma pathogenesis are discussed.

Original languageEnglish (US)
Article number280
JournalViruses
Volume10
Issue number6
DOIs
StatePublished - Jun 1 2018

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Feline Immunodeficiency Virus
T-Cell Lymphoma
Cats
Tetradecanoylphorbol Acetate
Lymphocytes
Nucleic Acids
Lymphoma
Infection
Lymph Nodes
Pancytopenia
TATA Box
Euthanasia
Terminal Repeat Sequences
Anorexia
Response Elements
Reporter Genes
Intestines
In Situ Hybridization
Single Nucleotide Polymorphism
Weight Loss

Keywords

  • Cat
  • FAIDS (feline AIDS)
  • FIV (feline immunodeficiency virus)
  • Immunopathology
  • Lymphoma

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Multiple, independent T cell lymphomas arising in an experimentally FIV-infected cat during the terminal stage of infection. / Murphy, Brian G; Eckstrand, Christina; Castillo, Diego; Poon, Andre; Liepnieks, Molly; Harmon, Kristy; Moore, Peter F.

In: Viruses, Vol. 10, No. 6, 280, 01.06.2018.

Research output: Contribution to journalArticle

Murphy, Brian G ; Eckstrand, Christina ; Castillo, Diego ; Poon, Andre ; Liepnieks, Molly ; Harmon, Kristy ; Moore, Peter F. / Multiple, independent T cell lymphomas arising in an experimentally FIV-infected cat during the terminal stage of infection. In: Viruses. 2018 ; Vol. 10, No. 6.
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