Multiple-drug-resistant mutants of feline immunodeficiency virus selected with 2',3'-dideoxyinosine alone and in combination with 3'-azido-3'- deoxythymidine

J. M. Gobert, K. M. Remington, Qi Zhu Ya Qi Zhu, T. W. North

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Mutants of feline immunodeficiency virus (FIV) were selected in cell culture in the continuous presence of 10 μM (each) 3'-azido-3'- deoxythymidine (AZT) and 2',3'-dideoxyinosine (ddI). These mutants (AIR-1 and AIR-3) displayed a 13-fold resistance to AZT but had less than a 2-fold decrease in susceptibility to ddI. Interestingly, the AIR mutants were cross- resistant to phosphonoformate (PFA) and were hypersensitive to 2',3'- dideoxycytidine (ddC). Mutants of FIV were also selected in the presence of 10 μM ddI alone (DIS-1, DIS-2c), and these displayed a two- to fourfold decrease in susceptibility to ddL. Like the mutants selected with the combination of AZT plus ddI, DIS-1 and DIS-2c were cross-resistant to PFA and were hypersensitive to ddC. However, they remained as susceptible as wild- type FIV to AZT. Thus, the mutants selected with the combination of AZT plus ddI have phenotypes which reflect those obtained by selection with these drugs individually.

Original languageEnglish (US)
Pages (from-to)861-864
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume38
Issue number4
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology (medical)

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