Mutants of feline immunodeficiency virus (FIV) were selected in cell culture in the continuous presence of 10 μM (each) 3'-azido-3'- deoxythymidine (AZT) and 2',3'-dideoxyinosine (ddI). These mutants (AIR-1 and AIR-3) displayed a 13-fold resistance to AZT but had less than a 2-fold decrease in susceptibility to ddI. Interestingly, the AIR mutants were cross- resistant to phosphonoformate (PFA) and were hypersensitive to 2',3'- dideoxycytidine (ddC). Mutants of FIV were also selected in the presence of 10 μM ddI alone (DIS-1, DIS-2c), and these displayed a two- to fourfold decrease in susceptibility to ddL. Like the mutants selected with the combination of AZT plus ddI, DIS-1 and DIS-2c were cross-resistant to PFA and were hypersensitive to ddC. However, they remained as susceptible as wild- type FIV to AZT. Thus, the mutants selected with the combination of AZT plus ddI have phenotypes which reflect those obtained by selection with these drugs individually.
|Original language||English (US)|
|Number of pages||4|
|Journal||Antimicrobial Agents and Chemotherapy|
|State||Published - 1994|
ASJC Scopus subject areas
- Pharmacology (medical)