Abstract
MLL rearrangements were analysed in the blood of a patient receiving chemotherapy for diffuse large B-cell lymphoma using inverse polymerase chain reaction targeting exon 12, parallel sequencing and a custom algorithm design. Of thirteen MLL rearrangements detected, five were capable of generating MLL fusion genes, including MLL-MLLT3, the most common fusion in acute myeloid leukaemia (AML). Other fusions, all previously clinically unobserved, included MLL-NKD1, a fusion to the negative regulator of Wnt/β-catenin signaling, a pathway linked to leukaemic cell proliferation. The majority of the fusions exhibited clonal persistence from before treatment until 6 months post-chemotherapy, suggesting the fusions may confer a survival advantage to the mutant clone. MLL breakpoints were partly clustered at a specific location, indicating commonality in the process of their formation. Further, the same MLL breakpoint location exhibited a 50-100-fold increase in C to T transitions, consistent with attack by activation-induced cytidine deaminase (AICDA). As is also observed in AML and acute lymphoblastic leukaemia, in this single patient setting, MLL is capable of interacting with multiple fusion partners. This finding defines a discrete site of MLL susceptibility to fragmentation, linked to possible deregulation of AICDA function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 50-57 |
| Number of pages | 8 |
| Journal | British Journal of Haematology |
| Volume | 159 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 2012 |
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Keywords
- Activation induced cytidine deaminase
- C to T transition
- Clonal MLL fusion
- MLL rearrangement
- Therapy-related acute myeloid leukamia
ASJC Scopus subject areas
- Hematology
Cite this
Multiple clonal MLL fusions in a patient receiving CHOP-based chemotherapy. / Shih, Shyh Jen; Fass, Joseph; Buffalo, Vincent; Lin, Dawei; Singh, Sheetal P.; Diaz, Manuel O.; Vaughan, Andrew T M.
In: British Journal of Haematology, Vol. 159, No. 1, 10.2012, p. 50-57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiple clonal MLL fusions in a patient receiving CHOP-based chemotherapy
AU - Shih, Shyh Jen
AU - Fass, Joseph
AU - Buffalo, Vincent
AU - Lin, Dawei
AU - Singh, Sheetal P.
AU - Diaz, Manuel O.
AU - Vaughan, Andrew T M
PY - 2012/10
Y1 - 2012/10
N2 - MLL rearrangements were analysed in the blood of a patient receiving chemotherapy for diffuse large B-cell lymphoma using inverse polymerase chain reaction targeting exon 12, parallel sequencing and a custom algorithm design. Of thirteen MLL rearrangements detected, five were capable of generating MLL fusion genes, including MLL-MLLT3, the most common fusion in acute myeloid leukaemia (AML). Other fusions, all previously clinically unobserved, included MLL-NKD1, a fusion to the negative regulator of Wnt/β-catenin signaling, a pathway linked to leukaemic cell proliferation. The majority of the fusions exhibited clonal persistence from before treatment until 6 months post-chemotherapy, suggesting the fusions may confer a survival advantage to the mutant clone. MLL breakpoints were partly clustered at a specific location, indicating commonality in the process of their formation. Further, the same MLL breakpoint location exhibited a 50-100-fold increase in C to T transitions, consistent with attack by activation-induced cytidine deaminase (AICDA). As is also observed in AML and acute lymphoblastic leukaemia, in this single patient setting, MLL is capable of interacting with multiple fusion partners. This finding defines a discrete site of MLL susceptibility to fragmentation, linked to possible deregulation of AICDA function.
AB - MLL rearrangements were analysed in the blood of a patient receiving chemotherapy for diffuse large B-cell lymphoma using inverse polymerase chain reaction targeting exon 12, parallel sequencing and a custom algorithm design. Of thirteen MLL rearrangements detected, five were capable of generating MLL fusion genes, including MLL-MLLT3, the most common fusion in acute myeloid leukaemia (AML). Other fusions, all previously clinically unobserved, included MLL-NKD1, a fusion to the negative regulator of Wnt/β-catenin signaling, a pathway linked to leukaemic cell proliferation. The majority of the fusions exhibited clonal persistence from before treatment until 6 months post-chemotherapy, suggesting the fusions may confer a survival advantage to the mutant clone. MLL breakpoints were partly clustered at a specific location, indicating commonality in the process of their formation. Further, the same MLL breakpoint location exhibited a 50-100-fold increase in C to T transitions, consistent with attack by activation-induced cytidine deaminase (AICDA). As is also observed in AML and acute lymphoblastic leukaemia, in this single patient setting, MLL is capable of interacting with multiple fusion partners. This finding defines a discrete site of MLL susceptibility to fragmentation, linked to possible deregulation of AICDA function.
KW - Activation induced cytidine deaminase
KW - C to T transition
KW - Clonal MLL fusion
KW - MLL rearrangement
KW - Therapy-related acute myeloid leukamia
UR - http://www.scopus.com/inward/record.url?scp=84866318176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866318176&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.2012.09248.x
DO - 10.1111/j.1365-2141.2012.09248.x
M3 - Article
VL - 159
SP - 50
EP - 57
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 1
ER -