Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships.

K. R. Squire, R. Y. Chuang, S. J. Dunn, C. A. Dangler, M. T. Falbo, L. F. Chuang, Bennie Osburn

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.

Original languageEnglish (US)
Pages (from-to)1785-1788
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume47
Issue number8
StatePublished - Aug 1 1986
Externally publishedYes

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ASJC Scopus subject areas

  • veterinary(all)

Cite this

Squire, K. R., Chuang, R. Y., Dunn, S. J., Dangler, C. A., Falbo, M. T., Chuang, L. F., & Osburn, B. (1986). Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships. American Journal of Veterinary Research, 47(8), 1785-1788.