Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships.

K. R. Squire, R. Y. Chuang, S. J. Dunn, C. A. Dangler, M. T. Falbo, L. F. Chuang, Bennie Osburn

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.

Original languageEnglish (US)
Pages (from-to)1785-1788
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume47
Issue number8
StatePublished - Aug 1 1986
Externally publishedYes

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Bluetongue virus
genetic relationships
Genome
genome
serotypes
hybridization
Firearms
prototypes
Organism Cloning
molecular cloning

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Squire, K. R., Chuang, R. Y., Dunn, S. J., Dangler, C. A., Falbo, M. T., Chuang, L. F., & Osburn, B. (1986). Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships. American Journal of Veterinary Research, 47(8), 1785-1788.

Multiple bluetongue virus-cloned genetic probes : application to diagnostics and bluetongue virus genetic relationships. / Squire, K. R.; Chuang, R. Y.; Dunn, S. J.; Dangler, C. A.; Falbo, M. T.; Chuang, L. F.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 47, No. 8, 01.08.1986, p. 1785-1788.

Research output: Contribution to journalArticle

Squire, KR, Chuang, RY, Dunn, SJ, Dangler, CA, Falbo, MT, Chuang, LF & Osburn, B 1986, 'Multiple bluetongue virus-cloned genetic probes: application to diagnostics and bluetongue virus genetic relationships.', American Journal of Veterinary Research, vol. 47, no. 8, pp. 1785-1788.
Squire, K. R. ; Chuang, R. Y. ; Dunn, S. J. ; Dangler, C. A. ; Falbo, M. T. ; Chuang, L. F. ; Osburn, Bennie. / Multiple bluetongue virus-cloned genetic probes : application to diagnostics and bluetongue virus genetic relationships. In: American Journal of Veterinary Research. 1986 ; Vol. 47, No. 8. pp. 1785-1788.
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