Abstract
A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1785-1788 |
| Number of pages | 4 |
| Journal | American Journal of Veterinary Research |
| Volume | 47 |
| Issue number | 8 |
| State | Published - Aug 1 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- veterinary(all)
Cite this
Multiple bluetongue virus-cloned genetic probes : application to diagnostics and bluetongue virus genetic relationships. / Squire, K. R.; Chuang, R. Y.; Dunn, S. J.; Dangler, C. A.; Falbo, M. T.; Chuang, L. F.; Osburn, Bennie.
In: American Journal of Veterinary Research, Vol. 47, No. 8, 01.08.1986, p. 1785-1788.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiple bluetongue virus-cloned genetic probes
T2 - application to diagnostics and bluetongue virus genetic relationships.
AU - Squire, K. R.
AU - Chuang, R. Y.
AU - Dunn, S. J.
AU - Dangler, C. A.
AU - Falbo, M. T.
AU - Chuang, L. F.
AU - Osburn, Bennie
PY - 1986/8/1
Y1 - 1986/8/1
N2 - A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.
AB - A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0022763611&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022763611&partnerID=8YFLogxK
M3 - Article
C2 - 3019191
AN - SCOPUS:0022763611
VL - 47
SP - 1785
EP - 1788
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 8
ER -