Abstract
A shotgun-cloning method incorporating all 10 bluetongue virus genome segments can simultaneously produce complete and partial copies of any of the genome segments. We report here 4 different cloned probes derived from 3 genome segments and individually defined by different hybridization recognition capabilities. One probe hybridized strongly with all 5 United States prototype strains of the 5 different bluetongue virus (BTV) serotypes existing in the United States and, as such, is a strong candidate for a broad BTV diagnostic probe in the United States. Another probe derived from genome segment 2 of BTV-17 hybridized only with the BTV-17 prototypic serotype, thereby demonstrating serospecific hybridization diagnostic potential. The implications for diagnostic and genetic relationship studies on BTV, using various genetic probes, are discussed.
Original language | English (US) |
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Pages (from-to) | 1785-1788 |
Number of pages | 4 |
Journal | American Journal of Veterinary Research |
Volume | 47 |
Issue number | 8 |
State | Published - Aug 1 1986 |
Externally published | Yes |
ASJC Scopus subject areas
- veterinary(all)