Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke

Vivek Srinivasan, Emiri T. Mandeville, Anil Can, Francesco Blasi, Mihail Climov, Ali Daneshmand, Jeong Hyun Lee, Esther Yu, Harsha Radhakrishnan, Eng H. Lo, Sava Sakadžić, Katharina Eikermann-Haerter, Cenk Ayata

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties.

Original languageEnglish (US)
Article numbere71478
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 7 2013

Fingerprint

Optical tomography
tomography
Optical Coherence Tomography
stroke
Stroke
image analysis
Scattering
Cerebrovascular Circulation
Imaging techniques
Recovery
Wounds and Injuries
Blood
Histology
blood flow
Hemodynamics
Biomarkers
Medicine
Brain
Microscopic examination
autoregulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke. / Srinivasan, Vivek; Mandeville, Emiri T.; Can, Anil; Blasi, Francesco; Climov, Mihail; Daneshmand, Ali; Lee, Jeong Hyun; Yu, Esther; Radhakrishnan, Harsha; Lo, Eng H.; Sakadžić, Sava; Eikermann-Haerter, Katharina; Ayata, Cenk.

In: PLoS One, Vol. 8, No. 8, e71478, 07.08.2013.

Research output: Contribution to journalArticle

Srinivasan, V, Mandeville, ET, Can, A, Blasi, F, Climov, M, Daneshmand, A, Lee, JH, Yu, E, Radhakrishnan, H, Lo, EH, Sakadžić, S, Eikermann-Haerter, K & Ayata, C 2013, 'Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke', PLoS One, vol. 8, no. 8, e71478. https://doi.org/10.1371/journal.pone.0071478
Srinivasan, Vivek ; Mandeville, Emiri T. ; Can, Anil ; Blasi, Francesco ; Climov, Mihail ; Daneshmand, Ali ; Lee, Jeong Hyun ; Yu, Esther ; Radhakrishnan, Harsha ; Lo, Eng H. ; Sakadžić, Sava ; Eikermann-Haerter, Katharina ; Ayata, Cenk. / Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke. In: PLoS One. 2013 ; Vol. 8, No. 8.
@article{e076c2cd9c384f6d8b0640380780b631,
title = "Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke",
abstract = "Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties.",
author = "Vivek Srinivasan and Mandeville, {Emiri T.} and Anil Can and Francesco Blasi and Mihail Climov and Ali Daneshmand and Lee, {Jeong Hyun} and Esther Yu and Harsha Radhakrishnan and Lo, {Eng H.} and Sava Sakadžić and Katharina Eikermann-Haerter and Cenk Ayata",
year = "2013",
month = "8",
day = "7",
doi = "10.1371/journal.pone.0071478",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Multiparametric, Longitudinal Optical Coherence Tomography Imaging Reveals Acute Injury and Chronic Recovery in Experimental Ischemic Stroke

AU - Srinivasan, Vivek

AU - Mandeville, Emiri T.

AU - Can, Anil

AU - Blasi, Francesco

AU - Climov, Mihail

AU - Daneshmand, Ali

AU - Lee, Jeong Hyun

AU - Yu, Esther

AU - Radhakrishnan, Harsha

AU - Lo, Eng H.

AU - Sakadžić, Sava

AU - Eikermann-Haerter, Katharina

AU - Ayata, Cenk

PY - 2013/8/7

Y1 - 2013/8/7

N2 - Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties.

AB - Progress in experimental stroke and translational medicine could be accelerated by high-resolution in vivo imaging of disease progression in the mouse cortex. Here, we introduce optical microscopic methods that monitor brain injury progression using intrinsic optical scattering properties of cortical tissue. A multi-parametric Optical Coherence Tomography (OCT) platform for longitudinal imaging of ischemic stroke in mice, through thinned-skull, reinforced cranial window surgical preparations, is described. In the acute stages, the spatiotemporal interplay between hemodynamics and cell viability, a key determinant of pathogenesis, was imaged. In acute stroke, microscopic biomarkers for eventual infarction, including capillary non-perfusion, cerebral blood flow deficiency, altered cellular scattering, and impaired autoregulation of cerebral blood flow, were quantified and correlated with histology. Additionally, longitudinal microscopy revealed remodeling and flow recovery after one week of chronic stroke. Intrinsic scattering properties serve as reporters of acute cellular and vascular injury and recovery in experimental stroke. Multi-parametric OCT represents a robust in vivo imaging platform to comprehensively investigate these properties.

UR - http://www.scopus.com/inward/record.url?scp=84881347568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881347568&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0071478

DO - 10.1371/journal.pone.0071478

M3 - Article

C2 - 23940761

AN - SCOPUS:84881347568

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e71478

ER -