Multimode optical imaging of small animals: Development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. M. Khazenzon, E. Lindsley, J. Ljubimova, A. G. Nowatzyk, D. L. Farkas, Sebastian Wachsmann-Hogiu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6441
DOIs
StatePublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: Jan 22 2007Jan 24 2007

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
CountryUnited States
CitySan Jose, CA
Period1/22/071/24/07

Fingerprint

Nanoconjugates
Animals
Imaging techniques
Tumors
Transillumination
Fluorescence
Laser excitation
Signal detection
CCD cameras
Optical systems
Image analysis
Laser pulses
Lenses
Topology
Wavelength
Lasers

Keywords

  • Bioluminescence imaging
  • Fluorescence imaging
  • Fluorescence lifetime imaging
  • Hyperspectral imaging and analysis
  • Mouse model
  • Multi-mode imaging system
  • Nanoconstruct
  • Trans-illumination imaging

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hwang, J. Y., Moffatt-Blue, C., Equils, O., Fujita, M., Jeong, J., Khazenzon, N. M., ... Wachsmann-Hogiu, S. (2007). Multimode optical imaging of small animals: Development and applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6441). [644105] https://doi.org/10.1117/12.711582

Multimode optical imaging of small animals : Development and applications. / Hwang, J. Y.; Moffatt-Blue, C.; Equils, O.; Fujita, M.; Jeong, J.; Khazenzon, N. M.; Lindsley, E.; Ljubimova, J.; Nowatzyk, A. G.; Farkas, D. L.; Wachsmann-Hogiu, Sebastian.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007. 644105.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hwang, JY, Moffatt-Blue, C, Equils, O, Fujita, M, Jeong, J, Khazenzon, NM, Lindsley, E, Ljubimova, J, Nowatzyk, AG, Farkas, DL & Wachsmann-Hogiu, S 2007, Multimode optical imaging of small animals: Development and applications. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6441, 644105, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, San Jose, CA, United States, 1/22/07. https://doi.org/10.1117/12.711582
Hwang JY, Moffatt-Blue C, Equils O, Fujita M, Jeong J, Khazenzon NM et al. Multimode optical imaging of small animals: Development and applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441. 2007. 644105 https://doi.org/10.1117/12.711582
Hwang, J. Y. ; Moffatt-Blue, C. ; Equils, O. ; Fujita, M. ; Jeong, J. ; Khazenzon, N. M. ; Lindsley, E. ; Ljubimova, J. ; Nowatzyk, A. G. ; Farkas, D. L. ; Wachsmann-Hogiu, Sebastian. / Multimode optical imaging of small animals : Development and applications. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007.
@inproceedings{98d3301b596c4958893e89bda3ba50be,
title = "Multimode optical imaging of small animals: Development and applications",
abstract = "We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.",
keywords = "Bioluminescence imaging, Fluorescence imaging, Fluorescence lifetime imaging, Hyperspectral imaging and analysis, Mouse model, Multi-mode imaging system, Nanoconstruct, Trans-illumination imaging",
author = "Hwang, {J. Y.} and C. Moffatt-Blue and O. Equils and M. Fujita and J. Jeong and Khazenzon, {N. M.} and E. Lindsley and J. Ljubimova and Nowatzyk, {A. G.} and Farkas, {D. L.} and Sebastian Wachsmann-Hogiu",
year = "2007",
doi = "10.1117/12.711582",
language = "English (US)",
isbn = "0819465542",
volume = "6441",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Multimode optical imaging of small animals

T2 - Development and applications

AU - Hwang, J. Y.

AU - Moffatt-Blue, C.

AU - Equils, O.

AU - Fujita, M.

AU - Jeong, J.

AU - Khazenzon, N. M.

AU - Lindsley, E.

AU - Ljubimova, J.

AU - Nowatzyk, A. G.

AU - Farkas, D. L.

AU - Wachsmann-Hogiu, Sebastian

PY - 2007

Y1 - 2007

N2 - We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

AB - We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

KW - Bioluminescence imaging

KW - Fluorescence imaging

KW - Fluorescence lifetime imaging

KW - Hyperspectral imaging and analysis

KW - Mouse model

KW - Multi-mode imaging system

KW - Nanoconstruct

KW - Trans-illumination imaging

UR - http://www.scopus.com/inward/record.url?scp=34247390132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247390132&partnerID=8YFLogxK

U2 - 10.1117/12.711582

DO - 10.1117/12.711582

M3 - Conference contribution

AN - SCOPUS:34247390132

SN - 0819465542

SN - 9780819465542

VL - 6441

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -