Multimode optical imaging of small animals: Development and applications

J. Y. Hwang; C. Moffatt-Blue; O. Equils; M. Fujita; J. Jeong; N. M. Khazenzon; E. Lindsley; J. Ljubimova; A. G. Nowatzyk; D. L. Farkas; Sebastian Wachsmann-Hogiu

doi:10.1117/12.711582

Multimode optical imaging of small animals: Development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. M. Khazenzon, E. Lindsley, J. Ljubimova, A. G. Nowatzyk, D. L. Farkas, Sebastian Wachsmann-Hogiu

Pathology and Laboratory Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6441
DOIs	https://doi.org/10.1117/12.711582
State	Published - 2007
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country	United States
City	San Jose, CA
Period	1/22/07 → 1/24/07

Keywords

Bioluminescence imaging
Fluorescence imaging
Fluorescence lifetime imaging
Hyperspectral imaging and analysis
Mouse model
Multi-mode imaging system
Nanoconstruct
Trans-illumination imaging

ASJC Scopus subject areas

Engineering(all)

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Hwang, J. Y., Moffatt-Blue, C., Equils, O., Fujita, M., Jeong, J., Khazenzon, N. M., Lindsley, E., Ljubimova, J., Nowatzyk, A. G., Farkas, D. L., & Wachsmann-Hogiu, S. (2007). Multimode optical imaging of small animals: Development and applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6441). [644105] https://doi.org/10.1117/12.711582

Multimode optical imaging of small animals : Development and applications. / Hwang, J. Y.; Moffatt-Blue, C.; Equils, O.; Fujita, M.; Jeong, J.; Khazenzon, N. M.; Lindsley, E.; Ljubimova, J.; Nowatzyk, A. G.; Farkas, D. L.; Wachsmann-Hogiu, Sebastian.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007. 644105.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hwang, JY, Moffatt-Blue, C, Equils, O, Fujita, M, Jeong, J, Khazenzon, NM, Lindsley, E, Ljubimova, J, Nowatzyk, AG, Farkas, DL & Wachsmann-Hogiu, S 2007, Multimode optical imaging of small animals: Development and applications. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6441, 644105, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, San Jose, CA, United States, 1/22/07. https://doi.org/10.1117/12.711582

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