Abstract
We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6441 |
| DOIs | |
| State | Published - 2007 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007 |
Other
| Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/22/07 → 1/24/07 |
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Keywords
- Bioluminescence imaging
- Fluorescence imaging
- Fluorescence lifetime imaging
- Hyperspectral imaging and analysis
- Mouse model
- Multi-mode imaging system
- Nanoconstruct
- Trans-illumination imaging
ASJC Scopus subject areas
- Engineering(all)
Cite this
Multimode optical imaging of small animals : Development and applications. / Hwang, J. Y.; Moffatt-Blue, C.; Equils, O.; Fujita, M.; Jeong, J.; Khazenzon, N. M.; Lindsley, E.; Ljubimova, J.; Nowatzyk, A. G.; Farkas, D. L.; Wachsmann-Hogiu, Sebastian.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007. 644105.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multimode optical imaging of small animals
T2 - Development and applications
AU - Hwang, J. Y.
AU - Moffatt-Blue, C.
AU - Equils, O.
AU - Fujita, M.
AU - Jeong, J.
AU - Khazenzon, N. M.
AU - Lindsley, E.
AU - Ljubimova, J.
AU - Nowatzyk, A. G.
AU - Farkas, D. L.
AU - Wachsmann-Hogiu, Sebastian
PY - 2007
Y1 - 2007
N2 - We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.
AB - We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.
KW - Bioluminescence imaging
KW - Fluorescence imaging
KW - Fluorescence lifetime imaging
KW - Hyperspectral imaging and analysis
KW - Mouse model
KW - Multi-mode imaging system
KW - Nanoconstruct
KW - Trans-illumination imaging
UR - http://www.scopus.com/inward/record.url?scp=34247390132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247390132&partnerID=8YFLogxK
U2 - 10.1117/12.711582
DO - 10.1117/12.711582
M3 - Conference contribution
AN - SCOPUS:34247390132
SN - 0819465542
SN - 9780819465542
VL - 6441
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -