Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism

Mohammad A. Yaseen, Vivek Srinivasan, Iwona Gorczynska, James G. Fujimoto, David A. Boas, Sava Sakadžić

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Improving our understanding of brain function requires novel tools to observe multiple physiological parameters with high resolution in vivo. We have developed a multimodal imaging system for investigating multiple facets of cerebral blood flow and metabolism in small animals. The system was custom designed and features multiple optical imaging capabilities, including 2-photon and confocal lifetime microscopy, optical coherence tomography, laser speckle imaging, and optical intrinsic signal imaging. Here, we provide details of the system’s design and present in vivo observations of multiple metrics of cerebral oxygen delivery and energy metabolism, including oxygen partial pressure, microvascular blood flow, and NADH autofluorescence.

Original languageEnglish (US)
Article number248448
Pages (from-to)4994-5007
Number of pages14
JournalBiomedical Optics Express
Volume6
Issue number12
DOIs
StatePublished - Nov 20 2015

Fingerprint

Multimodal Imaging
Optical Devices
Optical Imaging
metabolism
blood flow
Energy Metabolism
oxygen metabolism
delivery
Cerebrovascular Circulation
Oxygen
Partial Pressure
Optical Coherence Tomography
oxygen
Photons
Confocal Microscopy
systems engineering
NAD
brain
partial pressure
optical communication

Keywords

  • Imaging systems
  • Multiple imaging
  • Nonlinear microscopy
  • Optical coherence tomography

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism. / Yaseen, Mohammad A.; Srinivasan, Vivek; Gorczynska, Iwona; Fujimoto, James G.; Boas, David A.; Sakadžić, Sava.

In: Biomedical Optics Express, Vol. 6, No. 12, 248448, 20.11.2015, p. 4994-5007.

Research output: Contribution to journalArticle

Yaseen, Mohammad A. ; Srinivasan, Vivek ; Gorczynska, Iwona ; Fujimoto, James G. ; Boas, David A. ; Sakadžić, Sava. / Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 12. pp. 4994-5007.
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