Multimodal optical imaging system for in vivo investigation of cerebral oxygen delivery and energy metabolism

Mohammad A. Yaseen, Vivek Srinivasan, Iwona Gorczynska, James G. Fujimoto, David A. Boas, Sava Sakadžić

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Improving our understanding of brain function requires novel tools to observe multiple physiological parameters with high resolution in vivo. We have developed a multimodal imaging system for investigating multiple facets of cerebral blood flow and metabolism in small animals. The system was custom designed and features multiple optical imaging capabilities, including 2-photon and confocal lifetime microscopy, optical coherence tomography, laser speckle imaging, and optical intrinsic signal imaging. Here, we provide details of the system’s design and present in vivo observations of multiple metrics of cerebral oxygen delivery and energy metabolism, including oxygen partial pressure, microvascular blood flow, and NADH autofluorescence.

Original languageEnglish (US)
Article number248448
Pages (from-to)4994-5007
Number of pages14
JournalBiomedical Optics Express
Volume6
Issue number12
DOIs
StatePublished - Nov 20 2015

Keywords

  • Imaging systems
  • Multiple imaging
  • Nonlinear microscopy
  • Optical coherence tomography

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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