Abstract
There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1956-1966 |
| Number of pages | 11 |
| Journal | ACS Biomaterials Science and Engineering |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 8 2019 |
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Keywords
- fluorescence lifetime imaging
- mesenchymal stem/stromal cell
- osteogenic differentiation
- osteogenic graft
- ultrasound backscatter
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts. / Harvestine, Jenna N.; Sheaff, Clay S.; Li, Cai; Haudenschild, Anne K.; Gionet-Gonzales, Marissa A.; Hu, Jerry C.; Athanasiou, Kyriacos A.; Marcu, Laura; Leach, Jonathan K.
In: ACS Biomaterials Science and Engineering, Vol. 5, No. 4, 08.04.2019, p. 1956-1966.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts
AU - Harvestine, Jenna N.
AU - Sheaff, Clay S.
AU - Li, Cai
AU - Haudenschild, Anne K.
AU - Gionet-Gonzales, Marissa A.
AU - Hu, Jerry C.
AU - Athanasiou, Kyriacos A.
AU - Marcu, Laura
AU - Leach, Jonathan K
PY - 2019/4/8
Y1 - 2019/4/8
N2 - There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts.
AB - There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts.
KW - fluorescence lifetime imaging
KW - mesenchymal stem/stromal cell
KW - osteogenic differentiation
KW - osteogenic graft
KW - ultrasound backscatter
UR - http://www.scopus.com/inward/record.url?scp=85064109630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064109630&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.9b00007
DO - 10.1021/acsbiomaterials.9b00007
M3 - Article
AN - SCOPUS:85064109630
VL - 5
SP - 1956
EP - 1966
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 4
ER -