Multilevel analyses of genetic differentiation in Anopheles gambiae s.s. reveal patterns of gene flow important for malaria-fighting mosquito projects

Frédéric Tripet, Guimogo Dolo, Gregory C Lanzaro

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Malaria control projects based on the introduction and spread of transgenes into mosquito populations depend on the extent of isolation between those populations. On the basis of the distribution of paracentric inversions, Anopheles gambiae has been subdivided into five subspecific chromosomal forms. Estimating gene flow between and within these forms An. gambiae presents a number of challenges. We compared patterns of genetic divergence (F ST) between sympatric populations of the Bamako and Mopti forms at five sites. We used microsatellite loci within the j inversion on chromosome 2, which is fixed in the Bamako form but absent in the Mopti form, and microsatellites on chromosome 3, a region void of inversions. Estimates of genetic diversity and FST's suggest genetic exchanges between forms for the third chromosome but little for the j inversion. These results suggest a role for the inversion in speciation. Extensive gene flow within forms among sites resulted in populations clustering according to form despite substantial gene flow between forms. These patterns underscore the low levels of current gene flow between chromosomal forms in this area of sympatry. Introducing refractoriness genes in areas of the genome void of inversions may facilitate their spread within forms but their passage between forms may prove more difficult than previously thought.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalGenetics
Volume169
Issue number1
DOIs
StatePublished - Jan 2005

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Anopheles gambiae
Multilevel Analysis
Gene Flow
Culicidae
Malaria
Microsatellite Repeats
Population
Sympatry
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Transgenes
Cluster Analysis
Chromosomes
Genome
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Multilevel analyses of genetic differentiation in Anopheles gambiae s.s. reveal patterns of gene flow important for malaria-fighting mosquito projects. / Tripet, Frédéric; Dolo, Guimogo; Lanzaro, Gregory C.

In: Genetics, Vol. 169, No. 1, 01.2005, p. 313-324.

Research output: Contribution to journalArticle

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