Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities

Jiansong Cheng, Hai Yu, Kam Lau, Shengshu Huang, Harshal A. Chokhawala, Yanhong Li, Vinod Kumar Tiwari, Xi Chen

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

CstII from bacterium Campylobacter jejuni strain OH4384 has been previously characterized as a bifunctional sialyltransferase having both α2,3-sialyltransferase (GM3 oligosaccharide synthase) and α2,8-sialyltransferase (GD3 oligosaccharide synthase) activities which catalyze the transfer of N-acetylneuraminic acid (Neu5Ac) from cytidine 5′-monophosphate (CMP)-Neu5Ac to C-3′ of the galactose in lactose and to C-8 of the Neu5Ac in 3′-sialyllactose, respectively (Gilbert M, Karwaski MF, Bernatchez S, Young NM, Taboada E, Michniewicz J, Cunningham AM, Wakarchuk WW. 2002. The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide. J Biol Chem. 277:327-337). We report here the characterization of a truncated CstII mutant (CstIIΔ32I53S) cloned from a synthetic gene whose codons are optimized for an Escherichia coli expression system. In addition to the α2,3- and α2,8-sialyltransferase activities reported before for the synthesis of GM3- and GD3-type oligosaccharides, respectively, the CstIIΔ32I53S has α2,8-sialyltransferase (GT3 oligosaccharide synthase) activity for the synthesis of GT3 oligosaccharide. It also has α2,8-sialidase (GD3 oligosaccharide sialidase) activity that catalyzes the specific cleavage of the α2,8-sialyl linkage of GD3-type oligosaccharides and α2,8-trans-sialidase (GD3 oligosaccharide trans-sialidase) activity that catalyzes the transfer of a sialic acid from a GD3 oligosaccharide to a different GM3 oligosaccharide (3′-sialyllactoside). The donor substrate specificity study of the CstIIΔ32I53S GD3 oligosaccharide synthase activity indicates that the enzyme is flexible in using different CMP-activated sialic acids and their analogs for the synthesis of GD3 oligosaccharides containing natural and nonnatural modifications at the terminal sialic acid.

Original languageEnglish (US)
Pages (from-to)686-697
Number of pages12
JournalGlycobiology
Volume18
Issue number9
DOIs
StatePublished - 2008

Fingerprint

Sialyltransferases
Campylobacter jejuni
Neuraminidase
Oligosaccharides
N-Acetylneuraminic Acid
Cytidine Monophosphate
Cytidine
trans-sialidase
Sialic Acids
Synthetic Genes
Gangliosides
Lactose
Biosynthesis
Substrate Specificity
Galactose
Pathogens
Codon
Escherichia coli

Keywords

  • CstII
  • Ganglioside
  • Sialidase
  • Sialyltransferase
  • Trans-sialidase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Multifunctionality of Campylobacter jejuni sialyltransferase CstII : Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. / Cheng, Jiansong; Yu, Hai; Lau, Kam; Huang, Shengshu; Chokhawala, Harshal A.; Li, Yanhong; Tiwari, Vinod Kumar; Chen, Xi.

In: Glycobiology, Vol. 18, No. 9, 2008, p. 686-697.

Research output: Contribution to journalArticle

Cheng, Jiansong ; Yu, Hai ; Lau, Kam ; Huang, Shengshu ; Chokhawala, Harshal A. ; Li, Yanhong ; Tiwari, Vinod Kumar ; Chen, Xi. / Multifunctionality of Campylobacter jejuni sialyltransferase CstII : Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. In: Glycobiology. 2008 ; Vol. 18, No. 9. pp. 686-697.
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