Multicolumn infusion of gene therapy cells into human brain tumors: Technical report

Nazer H. Qureshi, Krzysztof S. Bankiewicz, David N. Louis, Fred H. Hochberg, E. Antonio Chiocca, Griffith R. Harsh IV

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

OBJECTIVE: Effective gene therapy for brain tumors may require saturation of the tumors with tumoricidal doses of the therapeutic gene. Safe, precise, and efficient delivery of gene therapy vectors is required. Most reported cases of and published protocols for gene therapy for brain tumors involve freehand injection of retroviral vectorproducing cells (VPCs) into the brain. Major disadvantages of this method include the inaccuracy of hand-guided needle placement and limited control of injection parameters. These factors can result in failure to deliver the viral vectors to specifically targeted sites within the brain, extensive tissue disruption resulting from excessively forceful injection, and reflux of the injectate along the needle tract. METHODS: We describe a novel stereotactic strategy for saturating tumor volumes with tumoricidal doses of gene therapy vectors and a new, more precise method of infusing VPCs. With our new instrument, the multicolumn stereotactic infusion system, needle placement is stereotactically guided and both VPC infusion and needle withdrawal are mechanically controlled. RESULTS: This technique, which has been used effectively for six patients, permits precise deposition of columns of VPCs throughout the targeted tumor volume. CONCLUSION: This technique should facilitate saturation of tumors with tumoricidal doses of gene therapy vectors and should improve the results of gene therapy protocols that rely on intraparenchymal injection for delivery.

Original languageEnglish (US)
Pages (from-to)663-669
Number of pages7
JournalNeurosurgery
Volume46
Issue number3
DOIs
StatePublished - Mar 2000
Externally publishedYes

Keywords

  • Brain tumor
  • Gene therapy
  • Glioblastoma multiforme
  • Malignant glioma
  • Retroviral vectors
  • Vector-producing cells

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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