Multicenter study of whole-blood creatinine, total carbon dioxide content, and chemistry profiling for laboratory and point-of-care testing in critical care in the United States

Gerald J Kost, Huynh Truong Vu, Michael Inn, Richard DuPlantier, Martin Fleisher, Martin H. Kroll, John C. Spinosa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objectives: To introduce a creatinine biosensor and a total carbon dioxide content (TCO2) method for whole-blood measurements, to evaluate the clinical performance of a new transportable analyzer that simultaneously performs these two and six other tests (Na+, K+, Cl-, glucose, urea nitrogen, and hematocrit), and to assess the potential of the new analyzer for point-of-care testing in critical care by comparing results obtained by nonlaboratory personnel and by medical technologists. Design: Multicenter sites compared whole-blood measurements with the transportable analyzer to plasma measurements from the same specimens with local reference instruments. One site compared whole-blood results produced by nonlaboratory personnel vs. medical technologists and evaluated day-to-day and within-day precision at the point of care. Settings and Patients: Four medical centers in the United States. Venous and arterial specimens from 710 critically ill patients with a variety of diagnoses. Point-of-care testing in the emergency room and operating room. Results: The linear regression analyses at the four medical centers showed the following: creatinine (a) slope, 0.91 to 1.22, (b) y intercept, -0.07 to 0.15 mg/dL, and (c) r2, 0.77 to 1.00; and TCO2: (a) slope, 0.64 to 1.00, (b) y intercept, 1.36 to 9.6 mmol/L, and (c) r2, 0.52 to 0.72 (y(i), whole-blood analyses; x(i), plasma reference measurements). Bland-Altman plots also were used to assess multicenter creatinine and TCO2 results. Of the other analytes, K+, glucose, and urea nitrogen had the highest r2)-values. For the eight chemistry profile tests performed at the point of care (y(i), nonlaboratory personnel results; x(i), medical technologist results), the average value of r2 was 0.96 (SD 0.08) in the operating room and 0.96 (SD 0.06) in the emergency room, and mean paired differences (y(i) - x(l)) were not statistically or clinically significant. Precision was acceptable. Conclusions: The performance of the creatinine biosensor and the TCO2 method was acceptable for whole-blood samples. Comparisons of whole-blood results from the transportable analyzer and plasma results from the local reference instruments revealed analyte biases that may be attributed to differences between direct whole-blood analyses and indirect-diluted plasma measurements and other factors. Performance of nonlaboratory personnel and medical technologists was equivalent for point- of-care testing in critical care settings. The whole-blood analyzer should be useful when patient care demands immediate results.

Original languageEnglish (US)
Pages (from-to)2379-2389
Number of pages11
JournalCritical Care Medicine
Volume28
Issue number7
StatePublished - 2000

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Critical Care
Carbon Dioxide
Multicenter Studies
Creatinine
Medical Laboratory Personnel
Point-of-Care Systems
Biosensing Techniques
Operating Rooms
Urea
Hospital Emergency Service
Nitrogen
Glucose
Point-of-Care Testing
Hematocrit
Critical Illness
Linear Models
Patient Care
Regression Analysis

Keywords

  • And whole-blood analyzer
  • Biosensor
  • Carbon dioxide
  • Chloride
  • Emergency room
  • Glucose
  • Hematocrit
  • Operating room
  • Potassium
  • Precision sodium
  • Substrate- specific electrode
  • Urea nitrogen

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Multicenter study of whole-blood creatinine, total carbon dioxide content, and chemistry profiling for laboratory and point-of-care testing in critical care in the United States. / Kost, Gerald J; Vu, Huynh Truong; Inn, Michael; DuPlantier, Richard; Fleisher, Martin; Kroll, Martin H.; Spinosa, John C.

In: Critical Care Medicine, Vol. 28, No. 7, 2000, p. 2379-2389.

Research output: Contribution to journalArticle

Kost, Gerald J ; Vu, Huynh Truong ; Inn, Michael ; DuPlantier, Richard ; Fleisher, Martin ; Kroll, Martin H. ; Spinosa, John C. / Multicenter study of whole-blood creatinine, total carbon dioxide content, and chemistry profiling for laboratory and point-of-care testing in critical care in the United States. In: Critical Care Medicine. 2000 ; Vol. 28, No. 7. pp. 2379-2389.
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abstract = "Objectives: To introduce a creatinine biosensor and a total carbon dioxide content (TCO2) method for whole-blood measurements, to evaluate the clinical performance of a new transportable analyzer that simultaneously performs these two and six other tests (Na+, K+, Cl-, glucose, urea nitrogen, and hematocrit), and to assess the potential of the new analyzer for point-of-care testing in critical care by comparing results obtained by nonlaboratory personnel and by medical technologists. Design: Multicenter sites compared whole-blood measurements with the transportable analyzer to plasma measurements from the same specimens with local reference instruments. One site compared whole-blood results produced by nonlaboratory personnel vs. medical technologists and evaluated day-to-day and within-day precision at the point of care. Settings and Patients: Four medical centers in the United States. Venous and arterial specimens from 710 critically ill patients with a variety of diagnoses. Point-of-care testing in the emergency room and operating room. Results: The linear regression analyses at the four medical centers showed the following: creatinine (a) slope, 0.91 to 1.22, (b) y intercept, -0.07 to 0.15 mg/dL, and (c) r2, 0.77 to 1.00; and TCO2: (a) slope, 0.64 to 1.00, (b) y intercept, 1.36 to 9.6 mmol/L, and (c) r2, 0.52 to 0.72 (y(i), whole-blood analyses; x(i), plasma reference measurements). Bland-Altman plots also were used to assess multicenter creatinine and TCO2 results. Of the other analytes, K+, glucose, and urea nitrogen had the highest r2)-values. For the eight chemistry profile tests performed at the point of care (y(i), nonlaboratory personnel results; x(i), medical technologist results), the average value of r2 was 0.96 (SD 0.08) in the operating room and 0.96 (SD 0.06) in the emergency room, and mean paired differences (y(i) - x(l)) were not statistically or clinically significant. Precision was acceptable. Conclusions: The performance of the creatinine biosensor and the TCO2 method was acceptable for whole-blood samples. Comparisons of whole-blood results from the transportable analyzer and plasma results from the local reference instruments revealed analyte biases that may be attributed to differences between direct whole-blood analyses and indirect-diluted plasma measurements and other factors. Performance of nonlaboratory personnel and medical technologists was equivalent for point- of-care testing in critical care settings. The whole-blood analyzer should be useful when patient care demands immediate results.",
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T1 - Multicenter study of whole-blood creatinine, total carbon dioxide content, and chemistry profiling for laboratory and point-of-care testing in critical care in the United States

AU - Kost, Gerald J

AU - Vu, Huynh Truong

AU - Inn, Michael

AU - DuPlantier, Richard

AU - Fleisher, Martin

AU - Kroll, Martin H.

AU - Spinosa, John C.

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N2 - Objectives: To introduce a creatinine biosensor and a total carbon dioxide content (TCO2) method for whole-blood measurements, to evaluate the clinical performance of a new transportable analyzer that simultaneously performs these two and six other tests (Na+, K+, Cl-, glucose, urea nitrogen, and hematocrit), and to assess the potential of the new analyzer for point-of-care testing in critical care by comparing results obtained by nonlaboratory personnel and by medical technologists. Design: Multicenter sites compared whole-blood measurements with the transportable analyzer to plasma measurements from the same specimens with local reference instruments. One site compared whole-blood results produced by nonlaboratory personnel vs. medical technologists and evaluated day-to-day and within-day precision at the point of care. Settings and Patients: Four medical centers in the United States. Venous and arterial specimens from 710 critically ill patients with a variety of diagnoses. Point-of-care testing in the emergency room and operating room. Results: The linear regression analyses at the four medical centers showed the following: creatinine (a) slope, 0.91 to 1.22, (b) y intercept, -0.07 to 0.15 mg/dL, and (c) r2, 0.77 to 1.00; and TCO2: (a) slope, 0.64 to 1.00, (b) y intercept, 1.36 to 9.6 mmol/L, and (c) r2, 0.52 to 0.72 (y(i), whole-blood analyses; x(i), plasma reference measurements). Bland-Altman plots also were used to assess multicenter creatinine and TCO2 results. Of the other analytes, K+, glucose, and urea nitrogen had the highest r2)-values. For the eight chemistry profile tests performed at the point of care (y(i), nonlaboratory personnel results; x(i), medical technologist results), the average value of r2 was 0.96 (SD 0.08) in the operating room and 0.96 (SD 0.06) in the emergency room, and mean paired differences (y(i) - x(l)) were not statistically or clinically significant. Precision was acceptable. Conclusions: The performance of the creatinine biosensor and the TCO2 method was acceptable for whole-blood samples. Comparisons of whole-blood results from the transportable analyzer and plasma results from the local reference instruments revealed analyte biases that may be attributed to differences between direct whole-blood analyses and indirect-diluted plasma measurements and other factors. Performance of nonlaboratory personnel and medical technologists was equivalent for point- of-care testing in critical care settings. The whole-blood analyzer should be useful when patient care demands immediate results.

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KW - Carbon dioxide

KW - Chloride

KW - Emergency room

KW - Glucose

KW - Hematocrit

KW - Operating room

KW - Potassium

KW - Precision sodium

KW - Substrate- specific electrode

KW - Urea nitrogen

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