Abstract
Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 38-42 |
| Number of pages | 5 |
| Journal | Nature Cell Biology |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 14 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Multi-step control of spindle pole body duplication by cyclin-dependent kinase. / Haase, Steven B.; Winey, Mark; Reed, Steven I.
In: Nature Cell Biology, Vol. 3, No. 1, 14.02.2001, p. 38-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multi-step control of spindle pole body duplication by cyclin-dependent kinase
AU - Haase, Steven B.
AU - Winey, Mark
AU - Reed, Steven I.
PY - 2001/2/14
Y1 - 2001/2/14
N2 - Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.
AB - Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035146528&partnerID=8YFLogxK
U2 - 10.1038/35050543
DO - 10.1038/35050543
M3 - Article
C2 - 11146624
AN - SCOPUS:0035146528
VL - 3
SP - 38
EP - 42
JO - Nature Cell Biology
JF - Nature Cell Biology
SN - 1465-7392
IS - 1
ER -