Multi-step control of spindle pole body duplication by cyclin-dependent kinase

Steven B. Haase, Mark Winey, Steven I. Reed

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

Original languageEnglish (US)
Pages (from-to)38-42
Number of pages5
JournalNature Cell Biology
Volume3
Issue number1
DOIs
StatePublished - Feb 14 2001
Externally publishedYes

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Spindle Pole Bodies
Centrosome
Cyclin-Dependent Kinases
Chromosome Segregation
Saccharomycetales
Cyclins
Genomic Instability
Tumor Suppressor Genes
DNA Replication
Mitosis
Organelles
Yeasts
Phenotype
Mutation
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Multi-step control of spindle pole body duplication by cyclin-dependent kinase. / Haase, Steven B.; Winey, Mark; Reed, Steven I.

In: Nature Cell Biology, Vol. 3, No. 1, 14.02.2001, p. 38-42.

Research output: Contribution to journalArticle

Haase, Steven B. ; Winey, Mark ; Reed, Steven I. / Multi-step control of spindle pole body duplication by cyclin-dependent kinase. In: Nature Cell Biology. 2001 ; Vol. 3, No. 1. pp. 38-42.
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