Multi-step control of spindle pole body duplication by cyclin-dependent kinase

Steven B. Haase; Mark Winey; Steven I. Reed

doi:10.1038/35050543

Multi-step control of spindle pole body duplication by cyclin-dependent kinase

Steven B. Haase, Mark Winey, Steven I. Reed

Research output: Contribution to journal › Article

69 Scopus citations

Abstract

Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

Original language	English (US)
Pages (from-to)	38-42
Number of pages	5
Journal	Nature Cell Biology
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/35050543
State	Published - Feb 14 2001
Externally published	Yes

Fingerprint

ASJC Scopus subject areas

Cell Biology

Cite this

Haase, S. B., Winey, M., & Reed, S. I. (2001). Multi-step control of spindle pole body duplication by cyclin-dependent kinase. Nature Cell Biology, 3(1), 38-42. https://doi.org/10.1038/35050543

@article{a9055202b71d4bfc83bc1e6193a63ef7,

title = "Multi-step control of spindle pole body duplication by cyclin-dependent kinase",

abstract = "Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.",

author = "Haase, {Steven B.} and Mark Winey and Reed, {Steven I.}",

year = "2001",

month = feb

day = "14",

doi = "10.1038/35050543",

language = "English (US)",

volume = "3",

pages = "38--42",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Multi-step control of spindle pole body duplication by cyclin-dependent kinase

AU - Haase, Steven B.

AU - Winey, Mark

AU - Reed, Steven I.

PY - 2001/2/14

Y1 - 2001/2/14

N2 - Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

AB - Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

UR - http://www.scopus.com/inward/record.url?scp=0035146528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035146528&partnerID=8YFLogxK

U2 - 10.1038/35050543

DO - 10.1038/35050543

M3 - Article

C2 - 11146624

AN - SCOPUS:0035146528

VL - 3

SP - 38

EP - 42

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 1

ER -

Access to Document

10.1038/35050543