Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury

Rajesh N. Raman, Chris D. Pivetti, Rajendra Ramsamooj, Christoph Troppmann, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A major source of kidneys for transplant comes from deceased donors whose tissues have suffered an unknown amount of warm ischemia prior to retrieval, with no quantitative means to assess function before transplant. Toward addressing this need, non-contact monitoring of optical signatures in rat kidneys was performed in vivo during ischemia and reperfusion. Kidney autofluorescence images were captured under ultraviolet illumination (355 nm, 325 nm, and 266 nm) in order to provide information on related metabolic and non-metabolic response. In addition, light scattering images under 355 nm, 325 nm, and 266 nm, 500 nm illumination were monitored to report on changes in kidney optical properties giving rise to the observed autofluorescence signals during these processes. During reperfusion, various signal ratios were generated from the recorded signals and then parametrized. Time-dependent parameters derived from the ratio of autofluorescence under 355 nm excitation to that under 266 nm excitation, as well as from 500 nm scattered signal, were found capable of discriminating dysfunctional kidneys from those that were functional (p < 0.01) within hours of reperfusion. Kidney dysfunction was confirmed by subsequent survival study and histology following autopsy up to a week later. Physiologic changes potentially giving rise to the observed signals, including those in cellular metabolism, vascular response, tissue microstructure, and microenvironment chemistry, are discussed.

Original languageEnglish (US)
Title of host publicationOptical Biopsy XVI
Subtitle of host publicationToward Real-Time Spectroscopic Imaging and Diagnosis
PublisherSPIE
Volume10489
ISBN (Electronic)9781510614635
DOIs
StatePublished - Jan 1 2018
EventOptical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018 - San Francisco, United States
Duration: Jan 30 2018Jan 31 2018

Other

OtherOptical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018
CountryUnited States
CitySan Francisco
Period1/30/181/31/18

Fingerprint

Transplants
kidneys
Spectrum Analysis
Color
Lighting
Spectroscopy
Scattering
Tissue
Kidney
color
Histology
Wounds and Injuries
scattering
Metabolism
Light scattering
spectroscopy
Reperfusion
Rats
ischemia
Optical properties

Keywords

  • Autofluorescence
  • In vivo
  • Ischemia
  • Kidney
  • Light scattering
  • Reperfusion
  • Spectral imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Raman, R. N., Pivetti, C. D., Ramsamooj, R., Troppmann, C., & Demos, S. G. (2018). Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury. In Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis (Vol. 10489). [104890C] SPIE. https://doi.org/10.1117/12.2292293

Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury. / Raman, Rajesh N.; Pivetti, Chris D.; Ramsamooj, Rajendra; Troppmann, Christoph; Demos, Stavros G.

Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489 SPIE, 2018. 104890C.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Raman, RN, Pivetti, CD, Ramsamooj, R, Troppmann, C & Demos, SG 2018, Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury. in Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. vol. 10489, 104890C, SPIE, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018, San Francisco, United States, 1/30/18. https://doi.org/10.1117/12.2292293
Raman RN, Pivetti CD, Ramsamooj R, Troppmann C, Demos SG. Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury. In Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489. SPIE. 2018. 104890C https://doi.org/10.1117/12.2292293
Raman, Rajesh N. ; Pivetti, Chris D. ; Ramsamooj, Rajendra ; Troppmann, Christoph ; Demos, Stavros G. / Multi-color autofluorescence and scattering spectroscopy provides rapid assessment of kidney function following ischemic injury. Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489 SPIE, 2018.
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