Abstract
Canine distemper virus (CDV) infection of ferrets causes a disease characterized by fever, erythema, conjunctivitis and leukocytopenia, similar clinically to measles except for the fatal neurologic sequelae of CDV. We vaccinated juvenile ferrets twice at 4-week intervals by the intranasal or intraduodenal route with attenuated vaccinia (NYVAC) or canarypox virus (ALVAC) constructs containing the CDV hemagglutinin and fusion genes. Controls were vaccinated with the same vectors expressing rabies glycoprotein. Animals were challenged intranasally 4 weeks after the second vaccination with virulent CDV. Body weights, white blood cell (WBC) counts and temperatures were monitored and ferrets were observed daily for clinical signs of infection. WBCs were assayed for the presence of viral RNA by RT- PCR. Intranasally vaccinated animals survived challenge with no virologic or clinical evidence of infection. Vaccination by the intraduodenal route did not provide complete protection. All control animals developed typical distemper. Ferrets can be effectively protected against distemper by mucosal vaccination with poxvirus vaccines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 308-318 |
| Number of pages | 11 |
| Journal | Vaccine |
| Volume | 17 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 1999 |
| Externally published | Yes |
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Keywords
- Distemper
- Mucosal vaccination
- Recombinant poxviruses
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Mucosal vaccination with recombinant poxvirus vaccines protects ferrets against symptomatic CDV infection. / Welter, Janet; Taylor, Jill; Tartaglia, James; Paoletti, Enzo; Stephensen, Charles B.
In: Vaccine, Vol. 17, No. 4, 02.1999, p. 308-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mucosal vaccination with recombinant poxvirus vaccines protects ferrets against symptomatic CDV infection
AU - Welter, Janet
AU - Taylor, Jill
AU - Tartaglia, James
AU - Paoletti, Enzo
AU - Stephensen, Charles B.
PY - 1999/2
Y1 - 1999/2
N2 - Canine distemper virus (CDV) infection of ferrets causes a disease characterized by fever, erythema, conjunctivitis and leukocytopenia, similar clinically to measles except for the fatal neurologic sequelae of CDV. We vaccinated juvenile ferrets twice at 4-week intervals by the intranasal or intraduodenal route with attenuated vaccinia (NYVAC) or canarypox virus (ALVAC) constructs containing the CDV hemagglutinin and fusion genes. Controls were vaccinated with the same vectors expressing rabies glycoprotein. Animals were challenged intranasally 4 weeks after the second vaccination with virulent CDV. Body weights, white blood cell (WBC) counts and temperatures were monitored and ferrets were observed daily for clinical signs of infection. WBCs were assayed for the presence of viral RNA by RT- PCR. Intranasally vaccinated animals survived challenge with no virologic or clinical evidence of infection. Vaccination by the intraduodenal route did not provide complete protection. All control animals developed typical distemper. Ferrets can be effectively protected against distemper by mucosal vaccination with poxvirus vaccines.
AB - Canine distemper virus (CDV) infection of ferrets causes a disease characterized by fever, erythema, conjunctivitis and leukocytopenia, similar clinically to measles except for the fatal neurologic sequelae of CDV. We vaccinated juvenile ferrets twice at 4-week intervals by the intranasal or intraduodenal route with attenuated vaccinia (NYVAC) or canarypox virus (ALVAC) constructs containing the CDV hemagglutinin and fusion genes. Controls were vaccinated with the same vectors expressing rabies glycoprotein. Animals were challenged intranasally 4 weeks after the second vaccination with virulent CDV. Body weights, white blood cell (WBC) counts and temperatures were monitored and ferrets were observed daily for clinical signs of infection. WBCs were assayed for the presence of viral RNA by RT- PCR. Intranasally vaccinated animals survived challenge with no virologic or clinical evidence of infection. Vaccination by the intraduodenal route did not provide complete protection. All control animals developed typical distemper. Ferrets can be effectively protected against distemper by mucosal vaccination with poxvirus vaccines.
KW - Distemper
KW - Mucosal vaccination
KW - Recombinant poxviruses
UR - http://www.scopus.com/inward/record.url?scp=0345267358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345267358&partnerID=8YFLogxK
U2 - 10.1016/S0264-410X(98)00211-4
DO - 10.1016/S0264-410X(98)00211-4
M3 - Article
C2 - 9987168
AN - SCOPUS:0345267358
VL - 17
SP - 308
EP - 318
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 4
ER -