Mucosal vaccination with recombinant poxvirus vaccines protects ferrets against symptomatic CDV infection

Janet Welter, Jill Taylor, James Tartaglia, Enzo Paoletti, Charles B. Stephensen

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Canine distemper virus (CDV) infection of ferrets causes a disease characterized by fever, erythema, conjunctivitis and leukocytopenia, similar clinically to measles except for the fatal neurologic sequelae of CDV. We vaccinated juvenile ferrets twice at 4-week intervals by the intranasal or intraduodenal route with attenuated vaccinia (NYVAC) or canarypox virus (ALVAC) constructs containing the CDV hemagglutinin and fusion genes. Controls were vaccinated with the same vectors expressing rabies glycoprotein. Animals were challenged intranasally 4 weeks after the second vaccination with virulent CDV. Body weights, white blood cell (WBC) counts and temperatures were monitored and ferrets were observed daily for clinical signs of infection. WBCs were assayed for the presence of viral RNA by RT- PCR. Intranasally vaccinated animals survived challenge with no virologic or clinical evidence of infection. Vaccination by the intraduodenal route did not provide complete protection. All control animals developed typical distemper. Ferrets can be effectively protected against distemper by mucosal vaccination with poxvirus vaccines.

Original languageEnglish (US)
Pages (from-to)308-318
Number of pages11
JournalVaccine
Volume17
Issue number4
DOIs
StatePublished - Feb 1999
Externally publishedYes

Keywords

  • Distemper
  • Mucosal vaccination
  • Recombinant poxviruses

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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