Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV

Ulrike Schmidt, Jörg Beyer, Ulf Polster, Laurel J Gershwin, Ursula J. Buchholz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSVΔG) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSVΔG led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSVΔG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV.

Original languageEnglish (US)
Pages (from-to)12355-12359
Number of pages5
JournalJournal of Virology
Volume76
Issue number23
DOIs
StatePublished - Dec 2002
Externally publishedYes

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Bovine respiratory syncytial virus
glycoproteins
Immunization
Glycoproteins
immunization
Respiratory Syncytial Virus Vaccines
calves
Virus Attachment
Respiratory Syncytial Virus Infections
Virus Replication
virus replication
Neutralizing Antibodies
neutralizing antibodies
respiratory system
infection
Respiratory System
Mental Competency
vaccines
Viruses
viruses

ASJC Scopus subject areas

  • Immunology

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Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV. / Schmidt, Ulrike; Beyer, Jörg; Polster, Ulf; Gershwin, Laurel J; Buchholz, Ursula J.

In: Journal of Virology, Vol. 76, No. 23, 12.2002, p. 12355-12359.

Research output: Contribution to journalArticle

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