Mucosal correlates of isolated HIV semen shedding during effective antiretroviral therapy

P. M. Sheth, T. J. Yi, C. Kovacs, K. S. Kemal, R. B. Jones, B. Osborne, R. Pilon, C. La Porte, M. Ostrowski, T. Mazzulli, Harold Burger, Barbara Weiser, R. Kaul

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Effective antiretroviral therapy (ART) suppresses the blood HIV RNA viral load (VL) below the level of detection. However, some individuals intermittently shed HIV RNA in semen despite suppression of viremia, a phenomenon termed "isolated HIV semen shedding (IHS)". In a previously reported clinical study, we collected blood and semen samples from HIV-infected men for 6 months after ART initiation, and documented IHS at ≥ 1 visit in almost half of the participants, independent of ART regimen or semen drug levels. We now report the mucosal immune associations of IHS in these men. Blood and semen plasma cytokine levels were assayed by multiplex enzyme-linked immunosorbent assay, T-cell populations were evaluated by flow cytometry in freshly isolated blood and semen mononuclear cells, and semen cytomegalovirus (CMV) DNA levels were measured by PCR. Although IHS was not associated with altered blood or semen cytokine levels, the phenomenon was associated with a transient, dramatic increase in CD4+ and CD8+ T-cell activation that was restricted to the semen compartment. All participants were CMV infected, and although semen CMV reactivation was common despite ART, this was not associated with T-cell activation or IHS. Further elucidation of the causes of compartmentalized mucosal T-cell activation and IHS may have important public health implications.

Original languageEnglish (US)
Pages (from-to)248-257
Number of pages10
JournalMucosal Immunology
Volume5
Issue number3
DOIs
StatePublished - May 2012
Externally publishedYes

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Semen
HIV
Therapeutics
Cytomegalovirus
T-Lymphocytes
RNA
Cytokines
Viremia
Viral Load
Flow Cytometry
Public Health

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Sheth, P. M., Yi, T. J., Kovacs, C., Kemal, K. S., Jones, R. B., Osborne, B., ... Kaul, R. (2012). Mucosal correlates of isolated HIV semen shedding during effective antiretroviral therapy. Mucosal Immunology, 5(3), 248-257. https://doi.org/10.1038/mi.2012.1

Mucosal correlates of isolated HIV semen shedding during effective antiretroviral therapy. / Sheth, P. M.; Yi, T. J.; Kovacs, C.; Kemal, K. S.; Jones, R. B.; Osborne, B.; Pilon, R.; La Porte, C.; Ostrowski, M.; Mazzulli, T.; Burger, Harold; Weiser, Barbara; Kaul, R.

In: Mucosal Immunology, Vol. 5, No. 3, 05.2012, p. 248-257.

Research output: Contribution to journalArticle

Sheth, PM, Yi, TJ, Kovacs, C, Kemal, KS, Jones, RB, Osborne, B, Pilon, R, La Porte, C, Ostrowski, M, Mazzulli, T, Burger, H, Weiser, B & Kaul, R 2012, 'Mucosal correlates of isolated HIV semen shedding during effective antiretroviral therapy', Mucosal Immunology, vol. 5, no. 3, pp. 248-257. https://doi.org/10.1038/mi.2012.1
Sheth, P. M. ; Yi, T. J. ; Kovacs, C. ; Kemal, K. S. ; Jones, R. B. ; Osborne, B. ; Pilon, R. ; La Porte, C. ; Ostrowski, M. ; Mazzulli, T. ; Burger, Harold ; Weiser, Barbara ; Kaul, R. / Mucosal correlates of isolated HIV semen shedding during effective antiretroviral therapy. In: Mucosal Immunology. 2012 ; Vol. 5, No. 3. pp. 248-257.
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