Abstract
The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1007-1014 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - Molecular Cell Research |
| Volume | 1773 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2007 |
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Keywords
- Adhesion
- Mucins
- Placenta
- Uterus
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Biophysics
Cite this
MUC1 is involved in trophoblast transendothelial migration. / Thirkill, Twanda L.; Cao, Tim; Stout, Michael; Blankenship, Thomas N.; Barakat, Abdul; Douglas, Gordon C.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1773, No. 6, 06.2007, p. 1007-1014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MUC1 is involved in trophoblast transendothelial migration
AU - Thirkill, Twanda L.
AU - Cao, Tim
AU - Stout, Michael
AU - Blankenship, Thomas N.
AU - Barakat, Abdul
AU - Douglas, Gordon C
PY - 2007/6
Y1 - 2007/6
N2 - The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.
AB - The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.
KW - Adhesion
KW - Mucins
KW - Placenta
KW - Uterus
UR - http://www.scopus.com/inward/record.url?scp=34249672915&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249672915&partnerID=8YFLogxK
U2 - 10.1016/j.bbamcr.2007.04.006
DO - 10.1016/j.bbamcr.2007.04.006
M3 - Article
C2 - 17509701
AN - SCOPUS:34249672915
VL - 1773
SP - 1007
EP - 1014
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 6
ER -