MUC1 is involved in trophoblast transendothelial migration

Twanda L. Thirkill, Tim Cao, Michael Stout, Thomas N. Blankenship, Abdul Barakat, Gordon C Douglas

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.

Original languageEnglish (US)
Pages (from-to)1007-1014
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1773
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Adhesion
  • Mucins
  • Placenta
  • Uterus

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

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