TY - JOUR
T1 - mRNA variants encoding multiple forms of the high-affinity IgE receptor alpha subunit in transformed and nontransformed mast cells
AU - Robertson, M. W.
AU - Mehl, V. S.
AU - Richards, M. L.
AU - Liu, Fu-Tong
PY - 1991
Y1 - 1991
N2 - Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor α subunit (FcεRI-α) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one FcεRI-α mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine FcεRI-α genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.
AB - Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor α subunit (FcεRI-α) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one FcεRI-α mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine FcεRI-α genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.
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M3 - Article
C2 - 1839735
AN - SCOPUS:0026347553
VL - 96
SP - 289
EP - 295
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 4
ER -