Mousepox detected in a research facility

Case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum

Philippe Labelle, Nina E. Hahn, Jenelle K. Fraser, Lonnie V. Kendall, Melanie Ziman, Edward James, Nilabh Shastri, Stephen M Griffey

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An outbreak of mousepox in a research institution was caused by Ectromelia-contaminated mouse serum that had been used for bone marrow cell culture and the cells subsequently injected into the footpads of mice. The disease initially was diagnosed by identification of gross and microscopic lesions typical for Ectromelia infection, including foci of necrosis in the liver and spleen and eosinophilic intracytoplasmic inclusion bodies in the skin. The source of infection was determined by PCR analysis to be serum obtained from a commercial vendor. To determine whether viral growth in tissue culture was required to induce viral infection, 36 mice (BALB/cJ, C57BL/6J) were experimentally exposed intraperitoneally, intradermally (footpad), or intranasally to contaminated serum or bone marrow cell cultures using the contaminated serum in the culture medium. Mice were euthanized when clinical signs developed or after 12 wk. Necropsy, PCR of spleen, and serum ELISA were performed on all mice. Mice injected with cell cultures and their cage contacts developed mousepox, antibodies to Ectromelia, and lesions, whereas mice injected with serum without cells did not. Mouse antibody production, a tool commonly used to screen biologic materials for viral contamination, failed to detect active Ectromelia contamination in mouse serum.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalComparative Medicine
Volume59
Issue number2
StatePublished - Apr 2009

Fingerprint

Infectious Ectromelia
Ectromelia virus
research facilities
antibody formation
Viruses
Cell culture
Antibody Formation
case studies
Ectromelia
Antibodies
mice
Bone
Testing
Contamination
Serum
Research
Tissue culture
testing
Liver
Culture Media

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Mousepox detected in a research facility : Case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum. / Labelle, Philippe; Hahn, Nina E.; Fraser, Jenelle K.; Kendall, Lonnie V.; Ziman, Melanie; James, Edward; Shastri, Nilabh; Griffey, Stephen M.

In: Comparative Medicine, Vol. 59, No. 2, 04.2009, p. 180-186.

Research output: Contribution to journalArticle

Labelle, Philippe ; Hahn, Nina E. ; Fraser, Jenelle K. ; Kendall, Lonnie V. ; Ziman, Melanie ; James, Edward ; Shastri, Nilabh ; Griffey, Stephen M. / Mousepox detected in a research facility : Case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum. In: Comparative Medicine. 2009 ; Vol. 59, No. 2. pp. 180-186.
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