Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5

E. Anne Smith, Michael F Seldin, Lisa Martinez, Mark L. Watson, Goutam Ghosh Choudhury, Peter A. Lalley, Jacalyne Pierce, Stuart Aaronson, Jane Barker, Susan L. Naylor, Alan Y. Sakaguchi

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

Original languageEnglish (US)
Pages (from-to)4811-4815
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number11
StatePublished - Jun 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • General
  • Genetics

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    Smith, E. A., Seldin, M. F., Martinez, L., Watson, M. L., Choudhury, G. G., Lalley, P. A., Pierce, J., Aaronson, S., Barker, J., Naylor, S. L., & Sakaguchi, A. Y. (1991). Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5. Proceedings of the National Academy of Sciences of the United States of America, 88(11), 4811-4815.