Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5

E. Anne Smith, Michael F Seldin, Lisa Martinez, Mark L. Watson, Goutam Ghosh Choudhury, Peter A. Lalley, Jacalyne Pierce, Stuart Aaronson, Jane Barker, Susan L. Naylor, Alan Y. Sakaguchi

Research output: Contribution to journalArticle

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Abstract

The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

Original languageEnglish (US)
Pages (from-to)4811-4815
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number11
StatePublished - Jun 1 1991
Externally publishedYes

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Platelet-Derived Growth Factor Receptors
Chromosomes, Human, Pair 5
Mutation
Genes
Receptor Protein-Tyrosine Kinases
Metrorrhagia
Hybrid Cells
Pulsed Field Gel Electrophoresis
DNA Probes
Cricetulus
Haplotypes
X-Rays
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5. / Smith, E. Anne; Seldin, Michael F; Martinez, Lisa; Watson, Mark L.; Choudhury, Goutam Ghosh; Lalley, Peter A.; Pierce, Jacalyne; Aaronson, Stuart; Barker, Jane; Naylor, Susan L.; Sakaguchi, Alan Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 11, 01.06.1991, p. 4811-4815.

Research output: Contribution to journalArticle

Smith, EA, Seldin, MF, Martinez, L, Watson, ML, Choudhury, GG, Lalley, PA, Pierce, J, Aaronson, S, Barker, J, Naylor, SL & Sakaguchi, AY 1991, 'Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5', Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 11, pp. 4811-4815.
Smith, E. Anne ; Seldin, Michael F ; Martinez, Lisa ; Watson, Mark L. ; Choudhury, Goutam Ghosh ; Lalley, Peter A. ; Pierce, Jacalyne ; Aaronson, Stuart ; Barker, Jane ; Naylor, Susan L. ; Sakaguchi, Alan Y. / Mouse platelet-derived growth factor receptor α gene is deleted in W19H and patch mutations on chromosome 5. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 11. pp. 4811-4815.
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abstract = "The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.",
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AU - Smith, E. Anne

AU - Seldin, Michael F

AU - Martinez, Lisa

AU - Watson, Mark L.

AU - Choudhury, Goutam Ghosh

AU - Lalley, Peter A.

AU - Pierce, Jacalyne

AU - Aaronson, Stuart

AU - Barker, Jane

AU - Naylor, Susan L.

AU - Sakaguchi, Alan Y.

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N2 - The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

AB - The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting mutation W (encoded by the Kit protooncogene), patch (Ph), and recessive lethal (l) loci. The platelet-derived growth factor receptor α gene (PDGFRA) like Kit encodes a transmembrane receptor tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids and haplotype analysis in interspecific backcross mice, mouse Pdgfra was mapped to chromosome 5 in tight linkage with Kit. Hybridization of a PDGFRA probe to DNAs from W19H/+ heterozygous mice and patch heterozygous mice, and their wild-type littermates, demonstrated deletion of Pdgfra. Pulsed-field gel electrophoresis indicated that Kit and Pdgfra are linked on a 630-kilobase Mlu I DNA fragment. Thus the W19H deletion removes at least two receptor tyrosine kinases and the results suggest Pdgfra as a candidate for the Ph locus.

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