Abstract
The methylation state of endogenous mouse mammary tumor virus (MuMTV) proviral DNA was examined in normal mouse tissues. DNAs from various tissues were cleaved with the methylation-sensitive enzymes HhaI and HpaII and analyzed by Southern blotting. Tissue-specific proviral DNA methylation patterns were found in the BALB/c, C3H, C57BL, GR/A, and GR-Mtv-2- mouse strains. MuMTV proviral DNA was hypomethylated in DNAs from the spleens and testes of all strains examined. The GR/A mouse strain, which was most thoroughly studied, also showed hypomethylation of MuMTV proviral DNA in bone marrow and placental tissues. Analysis of RNAs extracted from GR/A liver, mammary tumor, testes, placenta, and spleen tissues demonstrated that MuMTV proviral hypomethylation need not reflect significant proviral transcription.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 66-71 |
| Number of pages | 6 |
| Journal | Journal of Virology |
| Volume | 49 |
| Issue number | 1 |
| State | Published - Jan 1 1984 |
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ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology
Cite this
Mouse mammary tumor virus : Specific methylation patterns of proviral DNA in normal mouse tissues. / Hu, W. S.; Fanning, T. G.; Cardiff, Robert.
In: Journal of Virology, Vol. 49, No. 1, 01.01.1984, p. 66-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mouse mammary tumor virus
T2 - Specific methylation patterns of proviral DNA in normal mouse tissues
AU - Hu, W. S.
AU - Fanning, T. G.
AU - Cardiff, Robert
PY - 1984/1/1
Y1 - 1984/1/1
N2 - The methylation state of endogenous mouse mammary tumor virus (MuMTV) proviral DNA was examined in normal mouse tissues. DNAs from various tissues were cleaved with the methylation-sensitive enzymes HhaI and HpaII and analyzed by Southern blotting. Tissue-specific proviral DNA methylation patterns were found in the BALB/c, C3H, C57BL, GR/A, and GR-Mtv-2- mouse strains. MuMTV proviral DNA was hypomethylated in DNAs from the spleens and testes of all strains examined. The GR/A mouse strain, which was most thoroughly studied, also showed hypomethylation of MuMTV proviral DNA in bone marrow and placental tissues. Analysis of RNAs extracted from GR/A liver, mammary tumor, testes, placenta, and spleen tissues demonstrated that MuMTV proviral hypomethylation need not reflect significant proviral transcription.
AB - The methylation state of endogenous mouse mammary tumor virus (MuMTV) proviral DNA was examined in normal mouse tissues. DNAs from various tissues were cleaved with the methylation-sensitive enzymes HhaI and HpaII and analyzed by Southern blotting. Tissue-specific proviral DNA methylation patterns were found in the BALB/c, C3H, C57BL, GR/A, and GR-Mtv-2- mouse strains. MuMTV proviral DNA was hypomethylated in DNAs from the spleens and testes of all strains examined. The GR/A mouse strain, which was most thoroughly studied, also showed hypomethylation of MuMTV proviral DNA in bone marrow and placental tissues. Analysis of RNAs extracted from GR/A liver, mammary tumor, testes, placenta, and spleen tissues demonstrated that MuMTV proviral hypomethylation need not reflect significant proviral transcription.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021339952&partnerID=8YFLogxK
M3 - Article
C2 - 6317895
AN - SCOPUS:0021339952
VL - 49
SP - 66
EP - 71
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 1
ER -