Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation.

Stephen W Barthold, A. L. Smith

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Three-week-old outbred mice were inoculated intranasally with a mildly pathogenic strain of mouse hepatitis virus (MHV-S). Tissues were analyzed for distribution of infectious virus, lesions, and viral antigen at intervals up to 49 days after inoculation. Sera were tested for neutralizing antibody to MHV-S. Within the first week of infection, virus was isolated from lung and brain of most mice and liver of one mouse, but not from blood, spleen, or intestine. Microscopic lesions consisted of mild olfactory mucosal necrosis, neuronal necrosis of olfactory bulbs and tracts, lymphoplasmacytic infiltrates and vacuolation in the brain, mild nonsuppurative pulmonary perivascular lymphocyte infiltration, focal interstitial pneumonia, and focal necrotizing hepatitis. The presence and distribution of MHV antigen, as determined by indirect immunofluorescence, correlated with virus recovery and acute lesions. No virus or antigen was demonstrable beyond day 7. Serum antibody was first detected on day 10, and titers peaked on day 28 after infection.

Original languageEnglish (US)
Pages (from-to)355-360
Number of pages6
JournalLaboratory Animal Science
Volume33
Issue number4
StatePublished - Aug 1983
Externally publishedYes

Fingerprint

Murine hepatitis virus
weanlings
Viruses
Olfactory Bulb
lesions (animal)
viruses
mice
Necrosis
blood serum
Antigens
Lung
necrosis
Viral Antigens
lungs
Interstitial Lung Diseases
Brain
Virus Diseases
Indirect Fluorescent Antibody Technique
antigens
Neutralizing Antibodies

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation. / Barthold, Stephen W; Smith, A. L.

In: Laboratory Animal Science, Vol. 33, No. 4, 08.1983, p. 355-360.

Research output: Contribution to journalArticle

@article{335bef2b239a41e7bfdfef7995d01c81,
title = "Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation.",
abstract = "Three-week-old outbred mice were inoculated intranasally with a mildly pathogenic strain of mouse hepatitis virus (MHV-S). Tissues were analyzed for distribution of infectious virus, lesions, and viral antigen at intervals up to 49 days after inoculation. Sera were tested for neutralizing antibody to MHV-S. Within the first week of infection, virus was isolated from lung and brain of most mice and liver of one mouse, but not from blood, spleen, or intestine. Microscopic lesions consisted of mild olfactory mucosal necrosis, neuronal necrosis of olfactory bulbs and tracts, lymphoplasmacytic infiltrates and vacuolation in the brain, mild nonsuppurative pulmonary perivascular lymphocyte infiltration, focal interstitial pneumonia, and focal necrotizing hepatitis. The presence and distribution of MHV antigen, as determined by indirect immunofluorescence, correlated with virus recovery and acute lesions. No virus or antigen was demonstrable beyond day 7. Serum antibody was first detected on day 10, and titers peaked on day 28 after infection.",
author = "Barthold, {Stephen W} and Smith, {A. L.}",
year = "1983",
month = "8",
language = "English (US)",
volume = "33",
pages = "355--360",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "4",

}

TY - JOUR

T1 - Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation.

AU - Barthold, Stephen W

AU - Smith, A. L.

PY - 1983/8

Y1 - 1983/8

N2 - Three-week-old outbred mice were inoculated intranasally with a mildly pathogenic strain of mouse hepatitis virus (MHV-S). Tissues were analyzed for distribution of infectious virus, lesions, and viral antigen at intervals up to 49 days after inoculation. Sera were tested for neutralizing antibody to MHV-S. Within the first week of infection, virus was isolated from lung and brain of most mice and liver of one mouse, but not from blood, spleen, or intestine. Microscopic lesions consisted of mild olfactory mucosal necrosis, neuronal necrosis of olfactory bulbs and tracts, lymphoplasmacytic infiltrates and vacuolation in the brain, mild nonsuppurative pulmonary perivascular lymphocyte infiltration, focal interstitial pneumonia, and focal necrotizing hepatitis. The presence and distribution of MHV antigen, as determined by indirect immunofluorescence, correlated with virus recovery and acute lesions. No virus or antigen was demonstrable beyond day 7. Serum antibody was first detected on day 10, and titers peaked on day 28 after infection.

AB - Three-week-old outbred mice were inoculated intranasally with a mildly pathogenic strain of mouse hepatitis virus (MHV-S). Tissues were analyzed for distribution of infectious virus, lesions, and viral antigen at intervals up to 49 days after inoculation. Sera were tested for neutralizing antibody to MHV-S. Within the first week of infection, virus was isolated from lung and brain of most mice and liver of one mouse, but not from blood, spleen, or intestine. Microscopic lesions consisted of mild olfactory mucosal necrosis, neuronal necrosis of olfactory bulbs and tracts, lymphoplasmacytic infiltrates and vacuolation in the brain, mild nonsuppurative pulmonary perivascular lymphocyte infiltration, focal interstitial pneumonia, and focal necrotizing hepatitis. The presence and distribution of MHV antigen, as determined by indirect immunofluorescence, correlated with virus recovery and acute lesions. No virus or antigen was demonstrable beyond day 7. Serum antibody was first detected on day 10, and titers peaked on day 28 after infection.

UR - http://www.scopus.com/inward/record.url?scp=0020805033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020805033&partnerID=8YFLogxK

M3 - Article

C2 - 6312189

AN - SCOPUS:0020805033

VL - 33

SP - 355

EP - 360

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 4

ER -