Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion

D. G. Brownstein, Stephen W Barthold

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Mouse hepatitis viral antigens were demonstrated by immunofluorescence in formalin- and Bouin's-fixed tissues processed routinely for histopathology followed by partial digestion with trypsin. Staining was superior in tissues fixed in formalin and was not diminished in tissue sections from paraffin blocks stored at room temperature as long as 2 years. The relative ease of this procedure and the commercial availability of reagents makes this a useful technique for the definitive diagnosis of mouse hepatitis virus infection.

Original languageEnglish (US)
Title of host publicationLaboratory Animal Science
Pages37-39
Number of pages3
Volume32
Edition1
StatePublished - 1982
Externally publishedYes

Fingerprint

Murine hepatitis virus
Trypsin
Formaldehyde
Fluorescent Antibody Technique
Digestion
Hepatitis Antigens
Viral Antigens
Virus Diseases
Paraffin
Staining and Labeling
Temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Brownstein, D. G., & Barthold, S. W. (1982). Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion. In Laboratory Animal Science (1 ed., Vol. 32, pp. 37-39)

Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion. / Brownstein, D. G.; Barthold, Stephen W.

Laboratory Animal Science. Vol. 32 1. ed. 1982. p. 37-39.

Research output: Chapter in Book/Report/Conference proceedingChapter

Brownstein, DG & Barthold, SW 1982, Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion. in Laboratory Animal Science. 1 edn, vol. 32, pp. 37-39.
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