Mouse eye gene microarrays for investigating ocular development and disease

Rafal Farjo; Jindan Yu; Mohammad I. Othman; Shigeo Yoshida; Sujata Sheth; Thomas M Glaser; Wolfgang Baehr; Anand Swaroop

doi:10.1016/S0042-6989(01)00219-X

Mouse eye gene microarrays for investigating ocular development and disease

Rafal Farjo, Jindan Yu, Mohammad I. Othman, Shigeo Yoshida, Sujata Sheth, Thomas M Glaser, Wolfgang Baehr, Anand Swaroop

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.

Original language	English (US)
Pages (from-to)	463-470
Number of pages	8
Journal	Vision Research
Volume	42
Issue number	4
DOIs	https://doi.org/10.1016/S0042-6989(01)00219-X
State	Published - 2002
Externally published	Yes

Keywords

Cellular pathways
Expression analysis
Functional genomics
Gene profile
Mouse eye mutants
Retinal degeneration

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1016/S0042-6989(01)00219-X

Cite this

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title = "Mouse eye gene microarrays for investigating ocular development and disease",

abstract = "Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.",

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T1 - Mouse eye gene microarrays for investigating ocular development and disease

AU - Farjo, Rafal

AU - Yu, Jindan

AU - Othman, Mohammad I.

AU - Yoshida, Shigeo

AU - Sheth, Sujata

AU - Glaser, Thomas M

AU - Baehr, Wolfgang

AU - Swaroop, Anand

PY - 2002

Y1 - 2002

N2 - Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.

AB - Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.

KW - Cellular pathways

KW - Expression analysis

KW - Functional genomics

KW - Gene profile

KW - Mouse eye mutants

KW - Retinal degeneration

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Mouse eye gene microarrays for investigating ocular development and disease

Abstract

Keywords

ASJC Scopus subject areas

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