Mouse eye gene microarrays for investigating ocular development and disease

Rafal Farjo, Jindan Yu, Mohammad I. Othman, Shigeo Yoshida, Sujata Sheth, Thomas M Glaser, Wolfgang Baehr, Anand Swaroop

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.

Original languageEnglish (US)
Pages (from-to)463-470
Number of pages8
JournalVision Research
Volume42
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Cellular pathways
  • Expression analysis
  • Functional genomics
  • Gene profile
  • Mouse eye mutants
  • Retinal degeneration

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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    Farjo, R., Yu, J., Othman, M. I., Yoshida, S., Sheth, S., Glaser, T. M., Baehr, W., & Swaroop, A. (2002). Mouse eye gene microarrays for investigating ocular development and disease. Vision Research, 42(4), 463-470. https://doi.org/10.1016/S0042-6989(01)00219-X