Motor neuron pathology in experimental autoimmune encephalomyelitis

Studies in THY1-YFP transgenic mice

P. G. Bannerman, A. Hahn, S. Ramirez, M. Morley, C. Bönnemann, S. Yu, G. X. Zhang, A. Rostami, David E Pleasure

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Using adult male C57BL/6 mice that express a yellow fluorescent protein transgene in their motor neurons, we induced experimental autoimmune encephalomyelitis (EAE) by immunization with myelin oligodendrocyte glycoprotein peptide 35-55 (MOG peptide) in complete Freund's adjuvant (CFA). Control mice of the same transgenic strain received CFA without MOG peptide. Early in the course of their illness, the EAE mice showed lumbosacral spinal cord inflammation, demyelination and axonal fragmentation. By 14 weeks post-MOG peptide, these abnormalities were much less prominent, but the mice remained weak and, as in patients with progressive multiple sclerosis, spinal cord atrophy had developed. There was no significant loss of lumbar spinal cord motor neurons in the MOG peptide-EAE mice. However, early in the course of the illness, motor neuron dendrites were disrupted and motor neuron expression of hypophosphorylated neurofilament-H (hypoP-NF-H) immunoreactivity was diminished. By 14 weeks post-MOG peptide, hypoP-NF-H expression had returned to normal, but motor neuron dendritic abnormalities persisted and motor neuron perikaryal atrophy had appeared. We hypothesize that these motor neuron abnormalities contribute to weakness in this form of EAE and speculate that similar motor neuron abnormalities are present in patients with progressive multiple sclerosis.

Original languageEnglish (US)
Pages (from-to)1877-1886
Number of pages10
JournalBrain
Volume128
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Autoimmune Experimental Encephalomyelitis
Motor Neurons
Transgenic Mice
Pathology
Peptides
Spinal Cord
Intermediate Filaments
Freund's Adjuvant
Multiple Sclerosis
Atrophy
Myelitis
Demyelinating Diseases
Dendrites
Transgenes
Inbred C57BL Mouse
Immunization
myelin oligodendrocyte glycoprotein (35-55)

Keywords

  • Axonal degeneration
  • Dendrite
  • Experimental autoimmune encephalomyelitis (EAE)
  • Motor neuron
  • Multiple sclerosis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Motor neuron pathology in experimental autoimmune encephalomyelitis : Studies in THY1-YFP transgenic mice. / Bannerman, P. G.; Hahn, A.; Ramirez, S.; Morley, M.; Bönnemann, C.; Yu, S.; Zhang, G. X.; Rostami, A.; Pleasure, David E.

In: Brain, Vol. 128, No. 8, 08.2005, p. 1877-1886.

Research output: Contribution to journalArticle

Bannerman, PG, Hahn, A, Ramirez, S, Morley, M, Bönnemann, C, Yu, S, Zhang, GX, Rostami, A & Pleasure, DE 2005, 'Motor neuron pathology in experimental autoimmune encephalomyelitis: Studies in THY1-YFP transgenic mice', Brain, vol. 128, no. 8, pp. 1877-1886. https://doi.org/10.1093/brain/awh550
Bannerman PG, Hahn A, Ramirez S, Morley M, Bönnemann C, Yu S et al. Motor neuron pathology in experimental autoimmune encephalomyelitis: Studies in THY1-YFP transgenic mice. Brain. 2005 Aug;128(8):1877-1886. https://doi.org/10.1093/brain/awh550
Bannerman, P. G. ; Hahn, A. ; Ramirez, S. ; Morley, M. ; Bönnemann, C. ; Yu, S. ; Zhang, G. X. ; Rostami, A. ; Pleasure, David E. / Motor neuron pathology in experimental autoimmune encephalomyelitis : Studies in THY1-YFP transgenic mice. In: Brain. 2005 ; Vol. 128, No. 8. pp. 1877-1886.
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